Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
isoform PpaC in complex with Mn2+, hanging drop vapor diffusion method, using 1.32 M ammonium citrate tribasic | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
R295A | inactive | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Staphylococcus aureus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | dependent on, the active site contains two catalytic Mn2+ binding sites | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Staphylococcus aureus | - |
2 phosphate | - |
? | |
diphosphate + H2O | Staphylococcus aureus Mu50 | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P65752 | - |
- |
Staphylococcus aureus Mu50 | P65752 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-IMAC HisTrap column chromatography and S-200 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Staphylococcus aureus | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Staphylococcus aureus Mu50 | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Staphylococcus aureus |
PpaC | - |
Staphylococcus aureus |
PPIase | - |
Staphylococcus aureus |