Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pyrococcus furiosus | |
Cu2+ | - |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.173 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
1.37 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
3.12 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
3.32 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
3.47 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
3.56 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
3.61 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
3.63 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
4.13 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
4.43 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
5.01 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
6.09 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
11.8 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
Mg2+ | best activating ion | Pyrococcus furiosus | |
Mn2+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
additional information | not activated by Zn2+ | Pyrococcus furiosus | |
Ni2+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21740 | - |
2 * 21740, calculated from amino acid sequence | Pyrococcus furiosus |
22000 | - |
2 * 22000, SDS-PAGE | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Pyrococcus furiosus | most specific substrate | 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U438 | - |
- |
Pyrococcus furiosus ATCC 43587 | Q8U438 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0559 | - |
with diphosphate as substrate, at pH 9.5 and 95°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
ADP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
ATP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
ATP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
CDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
CDP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
CTP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
CTP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
diphosphate + H2O | most specific substrate | Pyrococcus furiosus | 2 phosphate | - |
? | |
GDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
GTP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
GTP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
IDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
ITP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
triphosphate + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
UDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
UDP-glucose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
UTP + H2O | weakest substrate | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 22000, SDS-PAGE | Pyrococcus furiosus |
homodimer | 2 * 21740, calculated from amino acid sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Pyrococcus furiosus |
PPase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 100 | the enzyme activity increases sharply from 80°C to 95°C. A moderate decrease in activity is observed between 95 and 100°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 100 | the enzyme shows half-lives of about 6850, 2760 and 850 min at 85, 95 and 100°C in the presence of Mg2+, respectively, as well as 3940, 1900, and 490 min at 85, 95 and 100°C in the absence of Mg2+, respectively | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.7 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
27.5 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
29.7 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
39.9 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
51.3 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
57.3 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
91.7 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
91.7 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
94.2 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
99.1 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
116 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
226 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
1230 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | over 50% of the maximal activity is retained in moderately alkaline conditions (pH ranging from 7.5 to 11.0 at 95°C), while less than 15% of the maximal activity of the enzyme is retained at pH values over 12.0 | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.44 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
4.87 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
8.28 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
8.4 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
11.2 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
16.4 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
16.5 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
22.2 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
23.2 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
25.2 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
26.1 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
164 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
7100 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus |