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Literature summary for 3.6.1.1 extracted from
- Characterization of a family I-liked alkaline-resistant inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus furiosus for rapid determination of sugar nucleotidyltransferase at high temperature (2013), J. Mol. Catal. B, 98, 15-20.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Pyrococcus furiosus |  |
| Cu2+ | - |
Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.173 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 1.37 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.12 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.32 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.47 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.56 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.61 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.63 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 4.13 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 4.43 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 5.01 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 6.09 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 11.8 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
| Mg2+ | best activating ion | Pyrococcus furiosus | |
| Mn2+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
| additional information | not activated by Zn2+ | Pyrococcus furiosus | |
| Ni2+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21740 | - |
2 * 21740, calculated from amino acid sequence | Pyrococcus furiosus |
| 22000 | - |
2 * 22000, SDS-PAGE | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Pyrococcus furiosus | most specific substrate | 2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8U438 | - |
- |
| Pyrococcus furiosus ATCC 43587 | Q8U438 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0559 | - |
with diphosphate as substrate, at pH 9.5 and 95°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| ADP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| ATP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| ATP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| CDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| CDP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| CTP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| CTP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| diphosphate + H2O | most specific substrate | Pyrococcus furiosus | 2 phosphate | - |
? | |
| GDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| GTP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| GTP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| IDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| ITP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| triphosphate + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| UDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| UDP-glucose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| UTP + H2O | weakest substrate | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 22000, SDS-PAGE | Pyrococcus furiosus |
| homodimer | 2 * 21740, calculated from amino acid sequence | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Pyrococcus furiosus |
| PPase | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | the enzyme activity increases sharply from 80°C to 95°C. A moderate decrease in activity is observed between 95 and 100°C | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | 100 | the enzyme shows half-lives of about 6850, 2760 and 850 min at 85, 95 and 100°C in the presence of Mg2+, respectively, as well as 3940, 1900, and 490 min at 85, 95 and 100°C in the absence of Mg2+, respectively | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.7 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 27.5 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 29.7 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 39.9 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 51.3 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 57.3 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 91.7 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 91.7 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 94.2 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 99.1 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 116 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 226 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 1230 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 11 | over 50% of the maximal activity is retained in moderately alkaline conditions (pH ranging from 7.5 to 11.0 at 95°C), while less than 15% of the maximal activity of the enzyme is retained at pH values over 12.0 | Pyrococcus furiosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.44 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 4.87 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 8.28 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 8.4 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 11.2 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 16.4 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 16.5 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 22.2 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 23.2 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 25.2 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 26.1 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 164 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 7100 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
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