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3.6.1.1: inorganic diphosphatase

This is an abbreviated version!
For detailed information about inorganic diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.1

Reaction

diphosphate
+
H2O
= 2 phosphate

Synonyms

acid PPase, AT727_13205, AtPPA1, AtPPsPase1, BT2127, CBS-PPase, CBS-PPase1, chloroplast inorganic pyrophosphatase 1, class A type PPA, cobalt-dependent inorganic pyrophosphatase, CPE2055, cytosolic PPase, D1C enzyme, dhPPase, E-PPase, EcPpA, EcPPiase, ehPPase, family I inorganic pyrophosphatase, family I PPase, family I pyrophosphatase, family II inorganic pyrophosphatase, family II PPase, H+-PPase, H+-pyrophosphatase, H+-translocating/vacuolar inorganic pyrophosphatase, Hpp1 protein, HVO_0729, HvPPA, hyperthermophilic inorganic pyrophosphatase, inorganic diphosphatase, inorganic PPase, inorganic pyrophosphatase, inorganic pyrophosphatase 1, inorganic pyrophosphatase-like protein, inorganic pyrophosphorylase, IPP, IPP1, Ipp1p, IPPase, LHPPase, M-PPase, manganese-dependent inorganic pyrophosphatase, MazG, MdPPa, membrane-integral inorganic pyrophosphatase, membrane-integral pyrophosphatase, miPPase, mitochondrial pyrophosphatase, Mn2+-bound canonical Family II PPase, More, Mtb PPiase, Mthe_0236, MtPPA1, MtPPiase, nucleotide-regulated pyrophosphatase, OVP1, p26.1a, p26.1b, PfPPase, PH1907, Pho-PPase, phospholysine phosphohistidine inorganic pyrophosphate phosphatase, phosphoprotein p26.1, PPA, PPA1, PpaC, PPase, PPase 1, PPase 2, ppase-1, PPase-2, PPase1, PPase2, PPase4, PPias, PPIase, proton-translocating inorganic pyrophosphatase, PYP-1, pyrophosphatase, pyrophosphatase, inorganic, Pyrophosphate phospho-hydrolase, pyrophosphate phospho-hydrolase 1, Pyrophosphate phosphohydrolase, Pyrophosphate-energized inorganic pyrophosphatase, RmPPase, Rv3628, siPPiase, soluble inorganic pyrophosphatase, soluble inorganic pyrophosphatase 2, soluble pyrophosphatase, soluble-type yeast PPase, sPPAse, ST2226, Tc-sPPase, TC004566, TcasGA2_TC004566, TgPPase, ThPP1, Tm-PPase, TM0913, V-PPase, vacuolar H(+)-PPase, vacuolar H(+)-pyrophosphatase, vacuolar H+-pyrophosphatase, vacuolar H+-translocating inorganic pyrophosphatase, vacuolar H+-translocating pyrophosphatase, vacuolar pyrophosphatase, VHP, VP1, VSP, VVPP1

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.1 inorganic diphosphatase

Temperature Stability

Temperature Stability on EC 3.6.1.1 - inorganic diphosphatase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
stable above 0.05 mM in Tris-HCl, Mg(CH3COO)2, 0.5 mM DTT, 2 h
0 - 25
-
stable to freezing, 0°C and thawing 25°
105
half-life about 50 min, recombinant enzyme
25
the enzyme remains active for 7 days
30
-
60 min, purified enzyme, 54% remaining activity
35
-
denaturation decreases with dithiothreitol
37
15 min, the mutant enzyme loses 70% activity in absence of Mg2+, half-life is 4.2 min
40
-
30 min, purified enzyme, 61% remaining activity
70 - 95
recombinant PhPPase, robust catalytic activity
74.3
T1/2 of the wild-type enzyme in absence of Mg2+
77.6
T1/2 of the wild-type enzyme in presence of Mg2+
85 - 100
the enzyme shows half-lives of about 6850, 2760 and 850 min at 85, 95 and 100°C in the presence of Mg2+, respectively, as well as 3940, 1900, and 490 min at 85, 95 and 100°C in the absence of Mg2+, respectively
89
-
melting temperature, presence of EDTA
90
4 h, 60% loss of activity
98
in the presence of 5 mM Mg2+ the midpoint of thermal denaturation is increased to 98°C
99
-
melting temperature, presence of Mg2+
additional information