Literature summary for 3.6.1.1 extracted from

He, R.; Yu, G.; Han, X.; Han, J.; Li, W.; Wang, B.; Huang, S.; Cheng, X.
ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress (2017), Plant Cell Rep., 36, 1929-1942 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene ThPP1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Oryza sativa subsp. japonica cv. Kitaake shoot and root using the transfection method via Agrobacterium tumefaciens strain GV3101, stable integration of the ThPP1 gene in the transgenic rice genome, construction of a ThPP1::16318hGFP protein expression vector and expression in in onion epidermal cells, quantitative RT-PCR expression analysis	Eutrema halophilum

Cloned (Comment)

Organism

gene ThPP1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Oryza sativa subsp. japonica cv. Kitaake shoot and root using the transfection method via Agrobacterium tumefaciens strain GV3101, stable integration of the ThPP1 gene in the transgenic rice genome, construction of a ThPP1::16318hGFP protein expression vector and expression in in onion epidermal cells, quantitative RT-PCR expression analysis

Eutrema halophilum

Protein Variants

Protein Variants	Comment	Organism
additional information	isolated soluble inorganic diphosphatase gene ThPP1 from Thellungiella halophila is transformed this gene into the Oryza sativa subsp. japonica cv. Kitaake to investigate the functional roles of the ThPP1 gene under saline-alkali stresses. Two independent transgenic lines of T3 generation are subjected to the alkali stress by NaHCO3 and Na2CO3, then the profiles of physiological changes and the transcriptome-based differentially expressions in the transgenic rice are evaluated. Rice transcriptome sequencing, phenotypes, overview. The transgenic rice shows beneficial physiological changes under alkali stress. The accumulations of sucrose and starch in the leaves of the transgenic lines are significantly higher than those of the wild-type rice plants	Eutrema halophilum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Eutrema halophilum	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	metal-binding protein	Eutrema halophilum

Organism

Organism	UniProt	Comment	Textmining
Eutrema halophilum	R4JUP5	i.e. Eutrema halophilum	-

Subunits

Subunits	Comment	Organism
?	x * 33500, about, sequence calculation	Eutrema halophilum
More	ThPP1 gene exhibits a typical structural characteristic of PPase family. ThPP1 protein is a hydrolase with the sites of metal binding protein, but having a tight single domain without the transmembrane structure	Eutrema halophilum

Synonyms

Synonyms	Comment	Organism
Hpp1 protein	UniProt	Eutrema halophilum
inorganic pyrophosphatase	-	Eutrema halophilum
inorganic pyrophosphorylase	-	Eutrema halophilum
ThPP1	-	Eutrema halophilum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Eutrema halophilum	sequence calculation	-	6.01

Expression

Organism	Comment	Expression
Eutrema halophilum	expression profiles of the ThPP1 gene in response to alkali stress, overview. Expression of the ThPP1 gene in Thellungiella halophila significantly increases under alkali treatment, at pH 8.5, the expression level of the ThPP1 gene is increased by 1.41fold, and at pH 9.0, the relative expression of the ThPP1 gene increased by 2.05fold compared to normal treatment	up

General Information

General Information	Comment	Organism
evolution	the ThPP1 gene was a PPase family I member. ThPP1 gene exhibits a typical structural characteristic of PPase family	Eutrema halophilum
additional information	ThPP1 protein is a hydrolase with the sites of metal binding protein, but having a tight single domain without the transmembrane structure	Eutrema halophilum
physiological function	inorganic pyrophosphorylase gene, ThPP1, modulates the accumulations of phosphate and osmolytes by upregulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress	Eutrema halophilum