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Literature summary for 3.6.1.1 extracted from
- Two soluble pyrophosphatases in Vibrio cholerae: transient redundancy or enduring cooperation? (2006), Biochemistry (Moscow), 71, 978-982.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| family I and family II isozymes, phylogenetic analysis, family II PPase is laterally transferred to the Vibrionales ancestor and partially degenerated due to functional redundancy, but nevertheless remains fixed as an adjunct to the family I enzyme, overexpression of PPase1 and PPase2 in Escherichia coli | Vibrio cholerae serotype O1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
diphosphate | pH 7.5, 25°C, recombinant PPase1, in presence of Mg2+ | Vibrio cholerae serotype O1 |  |
| 0.034 | - |
diphosphate | pH 7.5, 25°C, recombinant PPase2, in presence of Mn2+ | Vibrio cholerae serotype O1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | preferred by family I PPase, 20 mM | Vibrio cholerae serotype O1 | |
| Mn2+ | preferred by family II PPase, 50 mM | Vibrio cholerae serotype O1 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Vibrio cholerae serotype O1 | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
human pathogen, attenuated strain O1, family I and family II isozymes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the activity of PPase2 measured in the presence of Mn2+ is greater than that of PPase1 measured in the presence of Mg2+ | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Vibrio cholerae serotype O1 | 2 phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| family I PPase | - |
Vibrio cholerae serotype O1 |
| family II PPase | - |
Vibrio cholerae serotype O1 |
| PPase1 | - |
Vibrio cholerae serotype O1 |
| PPase2 | - |
Vibrio cholerae serotype O1 |
| soluble pyrophosphatase | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Vibrio cholerae serotype O1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 109 | - |
diphosphate | pH 7.5, 25°C, recombinant PPase1, in presence of Mg2+ | Vibrio cholerae serotype O1 | |
| 1600 | - |
diphosphate | pH 7.5, 25°C, recombinant PPase2, in presence of Mn2+ | Vibrio cholerae serotype O1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Vibrio cholerae serotype O1 |
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