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Literature summary for 3.6.1.1 extracted from
- Thermotoga maritima MazG protein has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities (2003), J. Biol. Chem., 278, 21408-21414 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E41Q/E42Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
| E45Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
| E61Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
| K118A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
| R97A/R98A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increases in the pyrophosphatase activity | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha,beta-methyleneadenosine triphosphate | cannot be hydrolyzed and blocks both NTPase and pyrophosphatase activities | Thermotoga maritima |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29728 | - |
mass spectrometry | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9X015 | - |
- |
| Thermotoga maritima DSM 3109 | Q9X015 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Thermotoga maritima | 2 phosphate | - |
? | |
| diphosphate + H2O | - |
Thermotoga maritima DSM 3109 | 2 phosphate | - |
? | |
| additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima | ? | - |
? | |
| additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima DSM 3109 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MazG | - |
Thermotoga maritima |
| pyrophosphatase | - |
Thermotoga maritima |
| TM0913 | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | 40°C: about 50% of maximal activity, 90°C: about 95% of maximal activity | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
stable at | Thermotoga maritima |
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Release 2023.1 (January 2023)
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