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Literature summary for 3.6.1.1 extracted from
- Characterization of two soluble inorganic pyrophosphatases from Arabidopsis thaliana (2007), Plant Sci., 172, 796-807.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged isozymes in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | high substrate inhibition at low levels of Mg2+ | Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | ordered binding of free Mg2+ and of the Mg-diphosphate complex. Both isozymes show similar catalytic constants and affinities for the Mg-diphosphate complex, while differed in their affinity for free Mg2+, kinetics, overview | Arabidopsis thaliana | |
| 0.059 | - |
diphosphate | pH 7.5, 25°C, isozyme PPa4 in a complex with Mg2+ | Arabidopsis thaliana | |
| 0.069 | - |
diphosphate | pH 7.5, 25°C, isozyme PPa1 in a complex with Mg2+ | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | isozyme PPa4 | Arabidopsis thaliana | 5737 | - |
| mitochondrion | isozyme PPa1, which contains an N-terminal mitochondrial target sequence with a putative cleavage site at Val31 | Arabidopsis thaliana | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, can be partially substituted by Mn2+, both isozymes show similar catalytic constants and affinities for the Mg-pyrophosphate complex, while differed in their affinity for free Mg2+, | Arabidopsis thaliana | |
| Mn2+ | can partially substitute for Mg2+ | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24484 | - |
x * 24484, isozyme PPa1, DNA sequence calculation, x * 24576, isozyme PPa4, DNA sequence calculation | Arabidopsis thaliana |
| 24576 | - |
x * 24484, isozyme PPa1, DNA sequence calculation, x * 24576, isozyme PPa4, DNA sequence calculation | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Arabidopsis thaliana | - |
2 phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LXC9 | PPase1; two isozymes PPase1 and PPase4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged isozymes from Escherichia coli by glutathione affinity chromatography, elution with ethylene glcol and Mg2+ | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | distribution of expression of isozymes during development in different tissues, overview | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Arabidopsis thaliana | 2 phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24484, isozyme PPa1, DNA sequence calculation, x * 24576, isozyme PPa4, DNA sequence calculation | Arabidopsis thaliana |
| More | circular dichroism spectrum, secondary structure of isozymes, overview | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Arabidopsis thaliana |
| PPase1 | - |
Arabidopsis thaliana |
| PPase4 | - |
Arabidopsis thaliana |
| siPPiase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.9 | - |
diphosphate | pH 7.5, 25°C, isozyme PPa4 | Arabidopsis thaliana | |
| 12.1 | - |
diphosphate | pH 7.5, 25°C, isozyme PPa1 | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | isozyme PPa4 | - |
5.26 |
| Arabidopsis thaliana | isozyme PPa1 | - |
5.73 |
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