Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, 20 mg/ml protien hanging drop vapour diffusion method, 3,8% PEG 4000, 0.1 M Na-acetate, pH 5.0-5.2, and 0.02 M MgCl2, asymmetric trimeric crystals, dimerization to hexamers, X-ray diffraction structure determination and analysis at 2.66 A resolution | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Pyrococcus horikoshii | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 | Pyrococcus horikoshii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diphosphate + H2O = 2 phosphate | active site structure | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Pyrococcus horikoshii | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | dimeric trimer, crystal structure | Pyrococcus horikoshii |
More | structural basis for the high thermostability | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
hyperthermophilic inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
Pho-PPase | - |
Pyrococcus horikoshii |
PPase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
optimal growth at 88°C | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
structural basis for the high thermostability | Pyrococcus horikoshii |