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Literature summary for 3.6.1.1 extracted from

  • Berger, S.; Welte, C.; Deppenmeier, U.
    Acetate activation in Methanosaeta thermophila: characterization of the key enzymes pyrophosphatase and acetyl-CoA synthetase (2012), Archaea, 2012, 315153.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanothrix thermoacetophila

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by nucleotides or its end product phosphate: neither addition of 0.75 mM AMP or 0.005 mM ADP nor addition of up to 1.5 mM phosphate led to a reduced reaction rate Methanothrix thermoacetophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 diphosphate pH 8.0, 37°C, with Mn2+ as metal cofactor Methanothrix thermoacetophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Methanothrix thermoacetophila
71400
-
 gel filtration Methanothrix thermoacetophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Methanothrix thermoacetophila the pyrophosphatase hydrolyzes the major part of diphosphate that is produced in the acetate activation reaction 2 phosphate
-
 ?
diphosphate + H2O Methanothrix thermoacetophila DSM 6194 the pyrophosphatase hydrolyzes the major part of diphosphate that is produced in the acetate activation reaction 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothrix thermoacetophila A0B5R0
-
-
Methanothrix thermoacetophila DSM 6194 A0B5R0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothrix thermoacetophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
157
-
 pH 8.0, 37°C, with Mg2+ as metal cofactor Methanothrix thermoacetophila
726
-
 pH 8.0, 37°C, with Mn2+ as metal cofactor Methanothrix thermoacetophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Methanothrix thermoacetophila 2 phosphate
-
 ?
diphosphate + H2O the pyrophosphatase hydrolyzes the major part of diphosphate that is produced in the acetate activation reaction Methanothrix thermoacetophila 2 phosphate
-
 ?
diphosphate + H2O
-
 Methanothrix thermoacetophila DSM 6194 2 phosphate
-
 ?
diphosphate + H2O the pyrophosphatase hydrolyzes the major part of diphosphate that is produced in the acetate activation reaction Methanothrix thermoacetophila DSM 6194 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Methanothrix thermoacetophila

Synonyms

Synonyms Comment Organism
Mthe_0236 locus name Methanothrix thermoacetophila

General Information

General Information Comment Organism
metabolism the soluble pyrophosphatasem RNA exists in great copy numbers in cells of Methanosaeta thermophila Methanothrix thermoacetophila