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3.5.4.1: cytosine deaminase

This is an abbreviated version!
For detailed information about cytosine deaminase, go to the full flat file.

Word Map on EC 3.5.4.1

Reaction

cytosine
+
H2O
=
Uracil
 +
NH3

Synonyms

A3DE, APOBEC1, APOBEC3, APOBEC3G, CD, CDA, CDase, codA, CodA protein, Cytosine aminohydrolase, cytosine deaminase, cytosine deaminase I, cytosine deaminase II, cytosine deaminase P, cytosine deaminase S, cytosine deaminase Y, Fca1p, FCY1, isocytosine deaminase, yCD, Zn2+CDase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.1 cytosine deaminase

Cloned

Cloned on EC 3.5.4.1 - cytosine deaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CD gene codA cloned into mammalian expression vector, WiDr cells, a human CRC cell line transformed
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CDase gene cloned into pBS+ vector, gentically engineered Escherichia coli strain that overexpresses the codBA operon on a multicopy plasmid
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codBA operon encoding cytosine permease and cytosine deaminase cloned
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development of an adenoviral vector for enzyme expression in cancer treatment by gene therapy, expression of the enzyme mutant D134A using this vector in human glioma cell lines D54MG, U87MG, and U251MG
-
enzyme expression in murine colorectal cancer cell line MC38
expressed in bone marrow-derived mesenchymal stem cells
expressed in Escherichia coli BL21(DE3) and BL21-RIL cells
-
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIPL cells
-
expression as fusion protein with uracil phosphoribosyltransferase in human pancreatic and colorectal cancer cell lines AsPC1, BxPC3, CaPan1, MIA PaCa2, Panc1, and HT-29, overview
expression as His-tagged enzyme in Escherichia coli strain BL21 and as selenomethionine-labeled enzyme in strain B834
-
expression in Clostridium sp.
-
expression in Escherichia coli TG1, pQE30Xa plasmid
expression in human cervical epithelial adenocarcinoma cell line HeLa, human pancreatic carcinoma cell line MIA PaCa-2, and human colon carcinoma cell lines WiDr and HT29
-
expression of a chimeric fusion protein consisting of cytosine deaminase and uracil phosphoribosyltransferase in human pancreatic cancer and glioma cell lines
-
expression of recombinant cytosine deaminase in Bifidobacterium breve I-53-8w using a shuttle plasmid Escherichia coli-Bifidobacterium, overview
-
expression of the chimeric mutant cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum, negative selection with 5-fluorocytosine
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expression of the enzyme cloned in a construct comprising an SV40 minimal promoter and nine copies of a hypoxia-responsive element in Y87-1 and Y87-2 cells confering the 5-fluorocytosine hydrolyzing activity to the transformed cells, negative control are Z87 and U-87 MG cells, overview
-
expression of the enzyme from a replication-incompetent vector, comprising the cytosine deaminase gene and the L-plastin promoter, in human hepatocellular carcinoma cell lines HepG2, Chang Liver, Huh-7 and SK-Hep-1, expression analysis in the different cell lines, overview
-
expression of the enzyme in human and murine cancer cell lines and tumors using a herpes simplex type 1 virus, HSV-1, by exchanging the viral gamma1_34.5 gene for the cytosine deaminase CD gene, method development for the oncolytic HSV vector in gene therapy, overview
-
expression of the enzyme in Rattus norvegicus, BDIX rats, in induced colorectal adenocarcinoma in a gene therapy approach
-
expression of the fusion gene encoding the extracellular and transmembrane domains of the human nerve growth factor receptor and the cytoplasmic portion of the yeast CD gene in murine 4T1 breast carcinoma cells as cytosolic protein, which renders the cells highly sensitive to treatment by 5-fluorocytosine prodrug
-
expression of the fusion protein HSV-1TKglyCD in HEK-293 cells and in the human lung cancer cell line NCIH460
expression of the His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein in Escherichia coli as soluble protein
-
expression of the recombinant fusion gene from adenoviral vector under control of the human telomerase reverse transcriptase promoter in murine syngeneic MBT-2 bladder tumor cells, overview
-
expression of wild-type and mutant enzymes in Escherichia coli enzyme-deficient strain GIA39(DE3), genetic complementation assays, construction of mammalian expression vectors, overview
expression vector with cytosine deaminase fused to monomeric DsRed (mDsRed), expression vector contains immediate early promoter of cytomegalovirus (CMV) and a hygromycin B resistance gene, transient transfection of COS-7 cells
FCA1 gene cloned from a genomic Candida albicans gene bank, constructed in the low-copy vector pMK122
-
FCY1 gene cloned and characterized
-
plasmid pBCMGSNeo/CD/CMV containing cytosine deaminase of Escherichia coli used for transfection of MC-38 cells
pMP201 vector and derivatives with aminoglycoside resistance gene, synthetic version of the cytosine deaminase gene (CodA), optimized for expression in Streptomyces species, generation of Streptomyces lividans TK24 protoplasts, transformation of Streptomyces lividans
stable transduction of human mesenchymal stem cells derived from adipose tissue (AT-MAS) with retroviral pST2 vector bearing yeast fusion cytosine deaminase, GP+E-86 cells and GP+envAM12 used as retroviral packaging mouse cell line, NIH-3T3 mouse fibroblast cells used for titration of viral inoculum
sucloning in strain JM109, construction of a shuttle plasmid Escherichia coli-Bifidobacterium longum, and expression in Bifidobacterium longum strain 105A, expression analysis
-
therapeutic CDase gene for human metastatic colorectal carcinoma
-