Application | Comment | Organism |
---|---|---|
medicine | oral 5-fluorocytosine in combination with selective delivery of CDase to cancerous cells by selectively introducing the bacterial CDase gene into the genome | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
CDase gene cloned into pBS+ vector, gentically engineered Escherichia coli strain that overexpresses the codBA operon on a multicopy plasmid | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | Fe2+CDase | Escherichia coli | |
H2O2 | only Fe2+CDase, Mn2+CDase, Co2+CDase and Zn2+CDase are not inhibited | Escherichia coli | |
o-phenanthroline | - |
Escherichia coli | |
Zn2+ | Fe2+CDase | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
cytosine | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Mn2+ reconstitutes apoenzyme after removal of Fe2+ | Escherichia coli | |
Fe2+ | - |
Escherichia coli | |
MnCl2 | Mn2+ reconstitutes apoenzyme after removal of Fe2+ | Escherichia coli | |
Zn2+ | Mn2+ reconstitutes apoenzyme after removal of Fe2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
4 * 48000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
+ 0.025 mM Cu(C2H3O2) | Escherichia coli |
2.6 | - |
+ 0.025 mM HgCl2 | Escherichia coli |
2.6 | - |
+ 0.025 mM Pb(C2H3O2) | Escherichia coli |
2.7 | - |
+ 0.025 mM CrCl2 | Escherichia coli |
2.9 | - |
apoCDase, + 0.025 SnCl2 | Escherichia coli |
3.1 | - |
+ 0.025 mM Cu(C2H3O2) | Escherichia coli |
3.2 | - |
+ 0.025 mM CaCl2 | Escherichia coli |
4 | - |
+ 0.025 mM NiCl2, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
5.6 | - |
+ 0.025 mM NiCl2 | Escherichia coli |
8.3 | - |
+ 0.025 mM Pb(C2H3O2), additional incubation of 0.025 mM Fe2+ | Escherichia coli |
11 | - |
+ 0.025 mM HgCl2, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
12 | - |
+ 0.025 mM Zn(C2H3O2), additional incubation with 0.025 mM Fe2+ | Escherichia coli |
13 | - |
+ 0.025 mM Zn(C2H3O2) | Escherichia coli |
21 | - |
+ 0.025 mM CoSO4, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
26 | - |
+ 0.025 mM CoSO4 | Escherichia coli |
27 | - |
- |
Escherichia coli |
45 | - |
+ 0.025 mM MnSO4 | Escherichia coli |
62 | - |
+ 0.025 mM MnSO4, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
65 | - |
+ 0.025 mM FeSO4, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
87 | - |
+ 0.025 mM FeSO4 | Escherichia coli |
87 | - |
+ 0.025 mM CrCl2, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
87 | - |
apoCDase, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
88 | - |
+ 0.025 mM CaCl2, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
94 | - |
+ 0.025 SnCl2, additional incubation with 0.025 mM Fe2+ | Escherichia coli |
Storage Stability | Organism |
---|---|
5°C, loses 40% of its activity after 2 weeks, lost activity can be restored by treatment with 0.05 mM FeSO4 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 48000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
73 | - |
extremely thermostable | Escherichia coli |