Cloned (Comment) | Organism |
---|---|
development of an adenoviral vector for enzyme expression in cancer treatment by gene therapy, expression of the enzyme mutant D134A using this vector in human glioma cell lines D54MG, U87MG, and U251MG | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D134A | site-directed mutagenesis, the mutant enzyme shows a higher affinity for cytosine than the wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | activation of the prodrug to the cytotoxic drug in cancer therapy by recombinant adenoviral vector expressed enzyme in human glioma cells, leads to increased 5-fluorouracil-mediated cell killing, overview | Escherichia coli | 5-fluorouracil + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Escherichia coli |