Application | Comment | Organism |
---|---|---|
pharmacology | cancer chemotherapy, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), biopanning assay | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TG1, pQE30Xa plasmid | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | Saccharomyces cerevisiae | recombinant protein of cytosine deaminase used as antigen for biopanning approach, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), binding of specific single chain fragment variable (scFv) with cytosine deaminase does not interfere with enzyme activity | 5-fluorouracil + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12178 | recombinant protein | - |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
selection and characterization of a human monoclonal antibody in single chain fragment (scFv) format for detection of expression of cytosine deaminase in GDEPT/ADEPT studies, selection and characterization of specific single chain fragment variable (scFv) antibody specific for cytosine deaminase, cytotoxic assay, amino acid sequence of the CDR3 regions of the selected antibody and genetic structure of phage antibody from ETH-2 library determined, human antibody generated by this method to detect cytosine deaminase in routinary laboratory techniques without interfering with its enzymatic function | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | recombinant protein of cytosine deaminase used as antigen for biopanning approach, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), binding of specific single chain fragment variable (scFv) with cytosine deaminase does not interfere with enzyme activity | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
yCD | - |
Saccharomyces cerevisiae |