Literature summary for 3.5.4.1 extracted from

Kucerova, L.; Matuskova, M.; Pastorakova, A.; Tyciakova, S.; Jakubikova, J.; Bohovic, R.; Altanerova, V.; Altaner, C.
Cytosine deaminase expressing human mesenchymal stem cells mediated tumour regression in melanoma bearing mice (2008), J. Gene Med., 10, 1071-1082.

Search for more literature for 3.5.4.1

Application

Application	Comment	Organism
pharmacology	applicability of gene-directed enzyme prodrug therapy using the capability of human adipose tissue-derived mesenchymal stem cells (AT-MSC) as cellular vehicles expressing cytosine deaminase, CDy-AT-MSC/5FC-system, analyzed in cell lines and xenografts	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
stable transduction of human mesenchymal stem cells derived from adipose tissue (AT-MAS) with retroviral pST2 vector bearing yeast fusion cytosine deaminase, GP+E-86 cells and GP+envAM12 used as retroviral packaging mouse cell line, NIH-3T3 mouse fibroblast cells used for titration of viral inoculum	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cytosine + H2O	Saccharomyces cerevisiae	-	uracil + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12178	recombinant protein	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	capability of human adipose tissue-derived mesenchymal stem cells (AT-MSC) as vehicles for gene-directed enzyme prodrug therapy, fusion product of cytosine deaminase derived from yeast in combination with systemic 5-fluorocytosine (5-Fc), expression and antitumoral effects of fusion constructs tested in cell lines and xenograft models, in vivo analysis in immunodeficient mouse model, regression in 89% of tumors achieved, therapeutic cell homing into subcutaneous melanoma and inhibition of tumor growth	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-fluorocytosine + H2O	recombinant cytosine deaminase in gene therapy, expression and function in transgenic cell lines and xenograft tumors	Saccharomyces cerevisiae	5-fluorouracil + NH3	-	?
cytosine + H2O	-	Saccharomyces cerevisiae	uracil + NH3	-	?

Synonyms

Synonyms	Comment	Organism
CD	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)