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Literature summary for 3.5.4.1 extracted from

  • Ou-Yang, F.; Lan, K.L.; Chen, C.T.; Liu, J.C.; Weng, C.L.; Chou, C.K.; Xie, X.; Hung, J.Y.; Wei, Y.; Hortobagyi, G.N.; Hung, M.C.
    Endostatin-cytosine deaminase fusion protein suppresses tumor growth by targeting neovascular endothelial cells (2006), Cancer Res., 66, 378-384.
    View publication on PubMed
Search for more literature for 3.5.4.1

Application

Application Comment Organism
medicine cytosine deaminase gene therapy combined with radiation treatment in breast carcinoma bone tumor, which is painful and causes osteolysis, is evaluated in a 4T1murine breast carcinoma model, overview Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of the fusion gene encoding the extracellular and transmembrane domains of the human nerve growth factor receptor and the cytoplasmic portion of the yeast CD gene in murine 4T1 breast carcinoma cells as cytosolic protein, which renders the cells highly sensitive to treatment by 5-fluorocytosine prodrug Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of a fusion gene encoding the extracellular and transmembrane domains of the human nerve growth factor receptor and the cytoplasmic portion of the yeast CD gene Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-fluorocytosine + H2O Saccharomyces cerevisiae activation of the prodrug by the recombinant fusion enzyme, overview 5-fluorouracil + NH3
-
 ?
cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?
additional information Saccharomyces cerevisiae cytosine deaminase gene therapy combined with radiation treatment in breast cancer is evaluated in a 4T1murine breast carcinoma model, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O activation of the prodrug by the recombinant fusion enzyme, overview Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
5-fluorocytosine + H2O activation of the prodrug Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?
additional information cytosine deaminase gene therapy combined with radiation treatment in breast cancer is evaluated in a 4T1murine breast carcinoma model, overview Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 in vivo assay at Saccharomyces cerevisiae