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Literature summary for 3.5.4.1 extracted from
- Cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cells in vitro (2007), Int. J. Radiat. Oncol. Biol. Phys., 67, 1538-1547.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | during hypoxia in Y87-1 and Y87-2 cells, a hypoxia-responsive element regulates expression of the cytosine deaminase gene and facilitates the conversion of 5-fluorocytosine to 5-fluorouracil | Saccharomyces cerevisiae |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | hypoxia-inducible cytosine deaminase/5-fluorocytosine therapy in combination with radiation therapy shows both a pronounced bystander effect and a radiosensitizing effect under hypoxic conditions | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the enzyme cloned in a construct comprising an SV40 minimal promoter and nine copies of a hypoxia-responsive element in Y87-1 and Y87-2 cells confering the 5-fluorocytosine hydrolyzing activity to the transformed cells, negative control are Z87 and U-87 MG cells, overview | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a fusion gene consisting of the SV40 minimal promoter, nine copies of a hypoxia-responsive element, and the yeast CD gene | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | Saccharomyces cerevisiae | recombinant enzyme in gene therapy in human glioblastoma cell lines Y87-1 and Y87-2 | 5-fluorouracil + NH3 | - |
? |  |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| additional information | Saccharomyces cerevisiae | cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cell lines Y87-1 and Y87-2 in vitro, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | recombinant enzyme in gene therapy in human glioblastoma cell lines Y87-1 and Y87-2 | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| additional information | cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cell lines Y87-1 and Y87-2 in vitro, overview | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
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