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3.4.21.4: trypsin

This is an abbreviated version!
For detailed information about trypsin, go to the full flat file.

Word Map on EC 3.4.21.4

Reaction

preferential cleavage: Arg-/-, Lys-/- =

Synonyms

acrosin, alpha-trypsin, anionic salmon trypsin, anionic trypsin, Anionic trypsinogen, AST, aT-I, aT-II, Atlantic cod trypsin I, beta-trypsin, BPT, Brain trypsinogen, BT, cationic trypsin, Cationic trypsinogen, cocoonase, CST, EC 3.4.4.4, EFE, EG-T, fibrinolytic enzyme, gamma-trypsin, GM-T, group III trypsin, mesotrypsin, Mesotrypsinogen, midgut-specific serine protease 1, midgut-specific serine protease 2, More, p23, parenzyme, parenzymol, PPT, pro23, pseudotrypsin, SET, SGT, sperm receptor hydrolase, ST-1, ST-2, ST-3, TI, TIIA, TIII, TLE, TLE I, TLE II, TR-P, TR-S, tripcellim, TRP, Try, TRY-3, TryI, TryII, TryIII, TRYP, trypsin 1, trypsin 3A1, trypsin 4, trypsin A, trypsin B, trypsin I, trypsin IV, trypsin type III, trypsin Y, trypsin-1, trypsin-2, trypsin-3, trypsin-4, trypsin-like enzyme, trypsin-like serine proteinase, tryptar, tryptase, trypure, TrypZean, type I trypsin, type IX pancreatic trypsin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.4 trypsin

Purification

Purification on EC 3.4.21.4 - trypsin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes
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3 enzyme form: anionic trypsin, cationic trypsin 1, cationic trypsin 2
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acetone precipitation and anhydrotrypsin-Sepharose column chromatography
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ammonium sulfate fractionation, acetone precipitation, benzamidine-Sepharose 6B affinity column chromatography, Sephadex G-75 gel filtration, and DEAE-Sepharose column chromatography
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ammonium sulfate fractionation, SBTI-Sepharose 4B column chromatography, and Sephadex G-75 gel filtration
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benzamidine-Sepharose 6B column chromatography
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benzamidine-Sepharose column chromatography
DEAE Sepharose FF column chromatography and Sephacryl S100 gel filtration
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double-mutant trypsinogen D189S/DELTA223
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enzyme form A and B
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further purification of the commercial preparation by gel filtration
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gamma-trypsin
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HzTrypsin-S and HzTrypsin-C
isoforms A, B, C
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isolation of a bovine trypsin isoform from commercial sample
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liquid-liquid extraction with polyethyleneglycol/sodium citrate aqueous two-phase systems, aqueous two-phase systems formed by PEG3350 and NaCit pH 5.2 show the best separation capability, the addition of NaCl up to a final concentration of 7% (w/w) and the decrease of top/bottom volume ratio to 0.1 leads to the recovery of 60% of pancreatic trypsin in a concentrated form in the top phase with a 3fold purification
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NAP-10 Sephadex G25 gel filtration and UNO Q6 column chromatography
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native enzyme 13.9fold to homogeneity by acetone precipitation, gel filtration, and anion exchange chromatography
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native enzyme 161.85fold by ammonium sulfate, ionic exchange chromatography, ultrafiltratrion, and benzamidine affinity chromatography
native enzyme 20fold from pyloric ceca by by ammonium sulfate precipitation, soybean trypsin inhibitor affinity and anion exchange chromatography to homogeneity
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native enzyme 24fold from pyloric ceca by gel filtration and diethylaminoethyl cellulose chromatography
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native enzyme 26.7fold by ammonium sulfate fractionation, and two different alternating steps of gel filtration and anion-exchange chromatography
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native enzyme 28fold from pyloric ceca to homogeneity by gel filtration
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native enzyme 33fold from pyloric ceca to homogeneity by gel filtration
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native enzyme 65fold from intestine by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
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native enzyme 8.5fold from viscera by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
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native enzyme 89fold from pyloric ceca by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration
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native enzyme from the pyloric caecum of tambaqui by heat treatment, ammonium sulfate fractionation, gel filtration, and 4-aminobenzamidine affinity chromatography
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native enzymes partially by aprotinin affinity chromatography, 51.2fold
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native trypsin A and B, 210.5fold and 118.3fold, respectively, from hepatopancreas to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
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native trypsin I by benzamidine affinity and anion exchange chromatography
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native trypsin isozymes A, B and C 5.95fold, 9.26fold, and 15.93fold, respectively, from viscera by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
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Ni-NTA Sepharose column chromatography and Resource S column chromatography
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Ni2+-TED-resin affinity column chromatography and soybean trypsin inhibitor affinity column chromatography
nickel-agarose affinity resin column chromatography and Superdex 200 gel filtration
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nickel-nitrilotriacetic acid affinity chromatography
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ProBond metal-chelating column chromatography and Mono Q column chromatoggraphy
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Q Sepharose Fast Flow column chromatography and Phenyl Sepharose Fast Flow column chromatography
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recombinant enzyme from Streptomyces lividans strain 1326 culture supernatant by cation exchange chromatography and dialysis
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recombinant enzyme from Streptomyces lividans strain 1326 culture supernatant by cation exchange chromatography and diialysis
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recombinant enzyme, using trypsin-specific benzamidine affinity column
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separation of serine proteases from a mixture by sunflower trypsin inhibitor-1 affinity chromatography
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Sephacryl S-200 gel filtration and Sephadex G-50 gel filtration
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Sephacryl S-200 gel filtration, Sephadex G-50 gel filtration and DEAE-cellulose column chromatography
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SP-Sepharose column chromatography
SP-Sepharose Fast Flow column chromatography and Arg-Sepharose 4B Fast Flow column chromatography
trypsin 1
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two isoforms: aT-I and aT-II
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