Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | 99% inhibition at 1 mM | Colossoma macropomum | |
EDTA | weak inhibition, 18% inhibition at 10 mM | Colossoma macropomum | |
additional information | no inhibition by N-alpha-tosyl-L-Phe-chloromethyl ketone, E-64, and O-fenantroline | Colossoma macropomum | |
N-alpha-tosyl-L-Lys-chloromethyl ketone | complete inhibition at 1 mM | Colossoma macropomum | |
PMSF | 73% inhibition at 1 mM | Colossoma macropomum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
Abz-FRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0006 | - |
Abz-KRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.001 | - |
Abz-RSFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.001 | - |
Abz-LRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0017 | - |
Abz-RYFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0017 | - |
Abz-RRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.00185 | - |
Abz-RHFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0029 | - |
Abz-VRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0031 | - |
Abz-RPFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.00325 | - |
Abz-RTFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0033 | - |
Abz-WRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0049 | - |
Abz-RNFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0056 | - |
Abz-YRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0068 | - |
Abz-HRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0069 | - |
Abz-ERFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0074 | - |
Abz-RQFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0074 | - |
Abz-NRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0081 | - |
Abz-RDFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0089 | - |
Abz-QRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0129 | - |
Abz-REFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0159 | - |
Abz-RGFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0163 | - |
Abz-GRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
0.0183 | - |
Abz-RVFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23900 | - |
x * 23900, SDS-PAGE | Colossoma macropomum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colossoma macropomum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from the pyloric caecum of tambaqui by heat treatment, ammonium sulfate fractionation, gel filtration, and 4-aminobenzamidine affinity chromatography | Colossoma macropomum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-ERFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-ER + FK-EDDnp | - |
? | |
Abz-FRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-FR + FK-EDDnp | - |
? | |
Abz-GRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-GR + FK-EDDnp | - |
? | |
Abz-HRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-HR + FK-EDDnp | - |
? | |
Abz-KRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-KR + FK-EDDnp | - |
? | |
Abz-LRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-LR + FK-EDDnp | - |
? | |
Abz-NRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-NR + FK-EDDnp | - |
? | |
Abz-QRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-QR + FK-EDDnp | - |
? | |
Abz-RDFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + DFK-EDDnp | - |
? | |
Abz-REFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + EFK-EDDnp | - |
? | |
Abz-RGFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + GFK-EDDnp | - |
? | |
Abz-RHFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + HFK-EDDnp | - |
? | |
Abz-RNFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + NFK-EDDnp | - |
? | |
Abz-RPFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + PFK-EDDnp | - |
? | |
Abz-RQFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + QFK-EDDnp | - |
? | |
Abz-RRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + RFK-EDDnp | - |
? | |
Abz-RSFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + SFK-EDDnp | - |
? | |
Abz-RTFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + TFK-EDDnp | - |
? | |
Abz-RVFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + VFK-EDDnp | - |
? | |
Abz-RYFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-R + YFK-EDDnp | - |
? | |
Abz-VRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-VR + FK-EDDnp | - |
? | |
Abz-WRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-WR + FK-EDDnp | - |
? | |
Abz-YRFK-EDDnp + H2O | - |
Colossoma macropomum | Abz-YR + FK-EDDnp | - |
? | |
additional information | the purified trypsin from Colossoma macropomum shows substrate specificity for arginine at P1, efficiently hydrolizing substrates with leucine and lysine at P2 and serine and arginine at P10 | Colossoma macropomum | ? | - |
? | |
N-benzoyl-DL-arginine 4-nitroanilide + H2O | a synthetic trypsin substrate | Colossoma macropomum | N-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Colossoma macropomum | N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23900, SDS-PAGE | Colossoma macropomum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
with substrate N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Colossoma macropomum |
70 | - |
with substrate N-benzoyl-DL-arginine 4-nitroanilide | Colossoma macropomum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, purified enzyme, 20% activity remaining with substrate N-benzoyl-DL-arginine 4-nitroanilide | Colossoma macropomum |
60 | - |
3 h, purified enzyme, 60% activity remaining with substrate N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Colossoma macropomum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Abz-RPFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
2.33 | - |
Abz-RDFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
4.59 | - |
Abz-RVFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
6.32 | - |
Abz-WRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
7.49 | - |
Abz-REFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
8.32 | - |
Abz-RHFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
8.87 | - |
Abz-FRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
9.15 | - |
Abz-RGFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
10.65 | - |
Abz-RYFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
12.64 | - |
Abz-QRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
13.31 | - |
Abz-RQFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
16.63 | - |
Abz-RSFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
16.63 | - |
Abz-RNFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
17.3 | - |
Abz-ERFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
18.3 | - |
Abz-RTFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
21.07 | - |
Abz-HRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
26.61 | - |
Abz-RRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
27.72 | - |
Abz-YRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
30.61 | - |
Abz-NRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
31.94 | - |
Abz-GRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
46.57 | - |
Abz-VRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
53.23 | - |
Abz-LRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
53.23 | - |
Abz-KRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11.5 | with substrate N-benzoyl-DL-arginine 4-nitroanilide | Colossoma macropomum |
9 | - |
with substrate N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Colossoma macropomum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
Abz-RPFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
250 | - |
Abz-RVFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
290 | - |
Abz-RDFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
570 | - |
Abz-RGFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
580 | - |
Abz-REFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
1420 | - |
Abz-QRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
1800 | - |
Abz-RQFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
1940 | - |
Abz-WRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
1960 | - |
Abz-GRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
2520 | - |
Abz-ERFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
3110 | - |
Abz-HRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
3380 | - |
Abz-RNFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
4120 | - |
Abz-NRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
4490 | - |
Abz-RHFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
4980 | - |
Abz-YRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
5630 | - |
Abz-RTFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
6400 | - |
Abz-RYFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
16220 | - |
Abz-VRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
16300 | - |
Abz-RSFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
16460 | - |
Abz-RRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
16680 | - |
Abz-FRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
52600 | - |
Abz-LRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum | |
91580 | - |
Abz-KRFK-EDDnp | pH 8.0, 25°C | Colossoma macropomum |