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Literature summary for 3.4.21.4 extracted from

  • Ulitina, N.N.; Khablyuk, V.V.; Proskuryakov, M.T.
    Purification and properties of serine proteinases from European catfish Silurus glanis L. pancreas (2005), Appl. Biochem. Microbiol., 41, 139-144.
No PubMed abstract available
Search for more literature for 3.4.21.4

General Stability

General Stability Organism
0.5 mM CaCl2 stabilizes the activity, especially at acidic pH-values Silurus glanis

Inhibitors

Inhibitors Comment Organism Structure
phenylmethylsulfonyl fluoride
-
 Silurus glanis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30100
-
 gel filtration, isoforms T1, T2, T3 Silurus glanis

Organism

Organism UniProt Comment Textmining
Silurus glanis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Silurus glanis

Source Tissue

Source Tissue Comment Organism Textmining
pancreas enzyme isoforms T1, T2, T3 Silurus glanis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
103
-
 isoform T2, 35°C Silurus glanis
109
-
 isoform T1, 35°C Silurus glanis
205
-
 isoform T3, 35°C Silurus glanis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Silurus glanis N-benzoyl-L-Arg + 4-nitroaniline
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 isoforms T1, T2 Silurus glanis
8.8
-
 isoform T3 Silurus glanis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.1 8.9 isoform T1 Silurus glanis
6.2 8.9 isoform T2 Silurus glanis
7.2 9.9 isoform T3 Silurus glanis

pI Value

Organism Comment pI Value Maximum pI Value
Silurus glanis isoform T1
-
 4.4
Silurus glanis isoform T2
-
 5.6
Silurus glanis isoform T3
-
 6.9