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Literature summary for 3.4.21.4 extracted from
- Comparative kinetic properties of alpha, beta and psi form of trypsin (1974), Eur. J. Biochem., 47, 295-302.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
benzoyl-Arg ethyl ester | alpha-trypsin | Bos taurus |  |
| 0.01 | - |
benzyloxycarbonyl-Lys p-nitrophenyl ester | alpha-trypsin | Bos taurus | |
| 0.017 | - |
tosyl-Arg methyl ester | alpha-trypsin | Bos taurus | |
| 0.0285 | - |
Benzyloxycarbonyl-Lys methyl ester | gamma-trypsin | Bos taurus | |
| 0.06 | - |
tosyl-Arg methyl ester | gamma-trypsin | Bos taurus | |
| 0.08 | - |
N-benzyloxycarbonyl-L-lysine benzyl ester | alpha-trypsin | Bos taurus | |
| 0.09 | - |
benzoyl-Arg ethyl ester | gamma-trypsin | Bos taurus | |
| 0.125 | - |
Benzyloxycarbonyl-Lys methyl ester | alpha-trypsin | Bos taurus | |
| 1.6 | - |
benzyloxycarbonyl-Lys p-nitrophenyl ester | gamma-trypsin | Bos taurus | |
| 22 | - |
N-benzyloxycarbonyl-L-lysine benzyl ester | gamma-trypsin | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gamma-trypsin | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-L-Arg amide + H2O | beta-trypsin is about 40% more active than alpha trypsin | Bos taurus | ? | - |
? | |
| benzyloxycarbonyl-L-Lys benzoyl ester + H2O | - |
Bos taurus | benzyloxycarbonyl-L-Lys + benzoate | - |
? | |
| Benzyloxycarbonyl-Lys methyl ester + H2O | - |
Bos taurus | Benzyloxycarbonyl-Lys + methanol | - |
? | |
| benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O | - |
Bos taurus | benzyloxycarbonyl-Lys + p-nitrophenol | - |
? | |
| N-Benzoyl-L-Arg ethyl ester + H2O | - |
Bos taurus | ? | - |
? | |
| N-benzyloxycarbonyl-L-lysine benzyl ester + H2O | - |
Bos taurus | N-benzyloxycarbonyl-L-lysine + toluene | - |
? | |
| p-nitrophenyl-p'-guanidino benzoate + H2O | beta-trypsin is more reactive than alpha-trypsin | Bos taurus | p-nitrophenol + p-guanidinobenzoate | - |
? | |
| p-tosyl-L-Arg methyl ester + H2O | - |
Bos taurus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
benzoyl-Arg ethyl ester | gamma-trypsin | Bos taurus | |
| 0.71 | - |
Benzyloxycarbonyl-Lys methyl ester | gamma-trypsin | Bos taurus | |
| 1.7 | - |
tosyl-Arg methyl ester | gamma-trypsin | Bos taurus | |
| 8 | - |
N-benzyloxycarbonyl-L-lysine benzyl ester | gamma-trypsin | Bos taurus | |
| 23 | - |
benzyloxycarbonyl-Lys p-nitrophenyl ester | gamma-trypsin | Bos taurus | |
| 26.6 | - |
benzoyl-Arg ethyl ester | alpha-trypsin | Bos taurus | |
| 76 | - |
tosyl-Arg methyl ester | alpha-trypsin | Bos taurus | |
| 80 | - |
Benzyloxycarbonyl-Lys methyl ester | alpha-trypsin | Bos taurus | |
| 82 | - |
benzyloxycarbonyl-Lys p-nitrophenyl ester | alpha-trypsin | Bos taurus | |
| 84 | - |
N-benzyloxycarbonyl-L-lysine benzyl ester | alpha-trypsin | Bos taurus | |
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