3.4.21.4: trypsin
This is an abbreviated version!
For detailed information about trypsin, go to the full flat file.
Reaction
preferential cleavage: Arg-/-, Lys-/-
=
Synonyms
acrosin, alpha-trypsin, anionic salmon trypsin, anionic trypsin, Anionic trypsinogen, AST, aT-I, aT-II, Atlantic cod trypsin I, beta-trypsin, BPT, Brain trypsinogen, BT, cationic trypsin, Cationic trypsinogen, cocoonase, CST, EC 3.4.4.4, EFE, EG-T, fibrinolytic enzyme, gamma-trypsin, GM-T, group III trypsin, mesotrypsin, Mesotrypsinogen, midgut-specific serine protease 1, midgut-specific serine protease 2, More, p23, parenzyme, parenzymol, PPT, pro23, pseudotrypsin, SET, SGT, sperm receptor hydrolase, ST-1, ST-2, ST-3, TI, TIIA, TIII, TLE, TLE I, TLE II, TR-P, TR-S, tripcellim, TRP, Try, TRY-3, TryI, TryII, TryIII, TRYP, trypsin 1, trypsin 3A1, trypsin 4, trypsin A, trypsin B, trypsin I, trypsin IV, trypsin type III, trypsin Y, trypsin-1, trypsin-2, trypsin-3, trypsin-4, trypsin-like enzyme, trypsin-like serine proteinase, tryptar, tryptase, trypure, TrypZean, type I trypsin, type IX pancreatic trypsin
ECTree
Application
Application on EC 3.4.21.4 - trypsin
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
food industry
-
the enzyme can be used as a possible biotechnological tool in the fish processing and food industries
analysis
-
application of biotinylated trypsin as a sensitive versatile probe for the detection and characterization of an ovine chondrocyte serine proteinase inhibitor using Western blotting
analysis
-
experimental system for the structural and physico-chemical analysis of factor Xa inhibitors in trypsin variants with factor Xa phenotype: E97N/Y99L, S190A, E97N/Y99L/S190A
analysis
-
the double mutant trypsinogen D189S/DELTA223 lacks trypsin-like activity but aquires a rather unique selectivity, it preferentially hydrolyses peptide bonds C-terminal to tyrosyl residues. This narrow specificity should be useful in peptide-analytical applications such as sequence-specific fragmentation of large proteins prior to sequencing
analysis
-
detection of enzyme hydrolytic activity by use of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate coupled to a chymotryptic petide obtained from hemoglobin on silica gel plate. Detection of 45 fmol of enzyme at 25°C within 1 min
analysis
comparison of five crystals of bovine trypsin obtained under analogous conditions. The Calpha and backbone atoms of the structures superpose very well. The occupancy of ligands in regions of low thermal motion is reproducible, whereas solvent molecules containing heavier atoms (such as sulfur) or those located on the surface can differ significantly. The coordination lengths of the calcium ion are conserved. A large proportion of the multiple conformations refine to similar occupancies and the residues adopt similar orientations. The protonation states of histidine residues and carboxylate moieties is consistent for all of the models. However, several features of residues or ligands located in flexible parts of the macromolecule may vary significantly, such as side-chain orientations and the occupancies of certain fragments
biotechnology
-
enzyme molecules are associated with the outer protein layer of rotavirus virions propagated in cell culture medium containing the enzyme. Enzyme is present only in triple-layer particles, not in double-layer particles. Enzyme associated with virions is inactive, activity is recovered only when the outer capsid is solubilized. Incorporation of trypsin into rotavirus particles may enhance its infectivity
biotechnology
recombinant Streptomyces erythraeus trypsin is fundamental to the use for proteomics applications
medicine
-
commercial-level production of trypsin in transgenic maize, the availability of this reagent should allow for the replacement of animal-derived trypsin in the processing of pharmaceutical proteins
medicine
-
use of enzyme and its regulators as markers of prognosis and disease activity in rheumatoid arthritis
medicine
-
trypsin is the chief culprit of severe acute pancreatitis-associated multiple organ dysfunction syndromes, the pancreatic tissue bleeding and necrosis is special pathological change in pancreas autodigestive effect from digestive enzymes such as trypsin in severe acute pancreatitis, the activated trypsin destroys the pancreas itself causing pancreatic tissue bleeding and necrosis, trypsin also destroys the vascular endothelial barrier leading to highly increased vascular permeability
medicine
role of trypsin as inducer of inflammation in skin via secretion of inflammatory mediators. King crab trypsin enhances the secretion ofIL-8, MMP-2 and MMP-9 by human skin keratinocytes. Exposure to proteases from the seafood may lead to inflammatory reactions in skin
medicine
-
role of trypsin as inducer of inflammation in skin via secretion of inflammatory mediators. Salmon trypsin enhances the secretion of IL-8 and MMP-2 by human skin keratinocytes. Exposure to proteases from the seafood may lead to inflammatory reactions in skin
medicine
dental plaque is a causative factor for oral disease and a potential reservoir for respiratory infection in the elderly. Therefore, there is a critical need for the development of effective methods to remove oral biofilm. Proteases reduce oral biofilm in vivo in elderly subjects. Tablets containing actinidin remove tongue coating in elderly subjects. Oral Actinomyces biofilm is significantly reduced by the proteases papain, actinidin and trypsin. Papain and trypsin effectively digest the major fimbrial proteins, FimP and FimA, from Actinomyces. Actinidin, papain and trypsin reduce multispecies biofilm that is reconstructed in vitro. Papain and trypsin inhibit formation of multispecies biofilm in vitro
synthesis
-
synthesis of benzoyl-Arg leucinamide by stabilized trypsin
synthesis
-
production of human insulin-threonine-ester, which can be simply converted into human insulin by hydrolysis with subsequent purification steps
synthesis
-
production of mono/di-arg insulin
synthesis
-
attachment of O-carboxymethyl-poly-beta-cyclodextrin with molecular weight of 13000 Da to surface of enzyme. resulting neoglycoenzyme retains high proteolytic and esterolytic activity, optimum temperature is increased by 10°C, enzyme is more resistant to thermal inactivation and to denaturation by sodiumdodecyl sulfate
synthesis
-
covalent immobilization of enzyme onto poly(methyl methacrylate)-co-(ethyl acrylate)-co-(acrylic acid) latex particles. Immobilized enzyme shows higher optimal temperature and pH-value than free form. Immobilized enzyme exhibits higher KM-value than free form and better chemical and thermal stability, it maintains 63% of initial activity after reusing ten times
synthesis
-
immobilisation of enzyme on Fe3O4 nanoparticles. The immobilized enzyme is slightly morestable than the free enzyme at 45°C. 55% of activity of immobilized trypsin remains at 44°C after 2 h and 90% after 120 days storage. 40% of its initial activity remains after eight times of successive reuse