Application | Comment | Organism |
---|---|---|
biotechnology | recombinant Streptomyces erythraeus trypsin is fundamental to the use for proteomics applications | Saccharopolyspora erythraea |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharopolyspora erythraea |
General Stability | Organism |
---|---|
in the so-called autolysis loop and shows no degradation after 96 h of incubation at 37°C | Saccharopolyspora erythraea |
Streptomyces erythraeus trypsin is resistant to autolytic degradation due to the absence of an arginine or lysine residue | Saccharopolyspora erythraea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Soybean trypsin inhibitor | - |
Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
N-alpha-benzoyl-L-Arg-p-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Saccharopolyspora erythraea | |
0.68 | - |
N-alpha-benzoyl-L-Lys-4-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Saccharopolyspora erythraea | |
5.7 | - |
N-alpha-benzoyl-L-Lys-4-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Sus scrofa | |
7.06 | - |
N-alpha-benzoyl-L-Arg-p-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23630 | - |
trypsin inhibitor purified mature enzyme, calculated from amino acid sequence | Saccharopolyspora erythraea |
28620 | - |
nickel purified His-tag-proenzyme, calculated from amino acid sequence | Saccharopolyspora erythraea |
35000 | - |
trypsin inhibitor purified mature enzyme, SDS-PAGE | Saccharopolyspora erythraea |
40000 | - |
nickel purified His-tag-proenzyme, SDS-PAGE | Saccharopolyspora erythraea |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | the recombinant enzyme retains about 40 and 20% activity in 0.1 and 0.3 M guanidine-HCl | Sus scrofa |
guanidine-HCl | the recombinant enzyme retains about 40 and 20% activity in 0.1 and 0.3 M guanidine-HCl | Saccharopolyspora erythraea |
urea | the recombinant enzyme retains approximately 50 and 20% of its activity in 2 and 4 M urea, respectively | Sus scrofa |
urea | the recombinant enzyme retains approximately 80 and 50% of its activity in 2 and 4 M urea, respectively | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q54137 | trypsin-like protease; synonym Saccharopolyspora erythraea | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-TED-resin affinity column chromatography and soybean trypsin inhibitor affinity column chromatography | Saccharopolyspora erythraea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O | - |
Sus scrofa | N-alpha-benzoyl-L-Arg + 4-nitroaniline | - |
? | |
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O | - |
Saccharopolyspora erythraea | N-alpha-benzoyl-L-Arg + 4-nitroaniline | - |
? | |
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Sus scrofa | N-alpha-benzoyl-L-Lys + p-nitroaniline | - |
? | |
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O | Streptomyces erythraeus trypsin has more than 1 order of magnitude greater amidase activity than mammalian trypsin | Saccharopolyspora erythraea | N-alpha-benzoyl-L-Lys + p-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
N-alpha-benzoyl-L-Lys-4-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Sus scrofa | |
1.04 | - |
N-alpha-benzoyl-L-Lys-4-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Saccharopolyspora erythraea | |
2.46 | - |
N-alpha-benzoyl-L-Arg-p-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Sus scrofa | |
5.73 | - |
N-alpha-benzoyl-L-Arg-p-nitroanilide | recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C | Saccharopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Sus scrofa |
7.5 | 9.5 | - |
Saccharopolyspora erythraea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Saccharopolyspora erythraea |
6.5 | 10 | - |
Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
the recombinant enzyme retains 25% of its maximum activity at pH 6.0 | Sus scrofa |
6 | - |
the recombinant enzyme retains 44% of its maximum activity at pH 6.0 | Saccharopolyspora erythraea |