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Literature summary for 3.4.21.4 extracted from

  • Jellouli, K.; Bougatef, A.; Daassi, D.; Balti, R.; Barkia, A.; Nasri, M.
    New alkaline trypsin from the intestine of Grey triggerfish (Balistes capriscus) with high activity at low temperature: purification and characterisation (2009), Food Chem., 116, 644-650.
    View publication on PubMed
Search for more literature for 3.4.21.4

General Stability

General Stability Organism
the enzyme activity is completely stable in presence of detergents, overview Balistes capriscus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 57% inhibition at 5 mM Balistes capriscus
additional information the enzyme activity is completely stable in presence of detergents, overview. No inhibition by EDTA, 2-nercaptoethanol, pepstatin A, Ca2+, Ba2+, Zn2+, Cu2+, Mg2+, Mn2+, K+, and Na+ Balistes capriscus
PMSF 80% inhibition at 1 mM, complete at 5 mM Balistes capriscus
Soybean trypsin inhibitor complete inhibition at 1 mg/ml Balistes capriscus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 Nalpha-benzoyl-DL-arginine 4-nitroanilide pH 10.5, 25°C Balistes capriscus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23200
-
 gel filtration and native PAGE Balistes capriscus

Organism

Organism UniProt Comment Textmining
Balistes capriscus
-
-
-

Oxidation Stability

Oxidation Stability Organism
about 87% of its initial activity remains after 1 h at 40°C in the presence of 1% sodium perborate, about 80% in the presence of 1% H2O2 Balistes capriscus

Purification (Commentary)

Purification (Comment) Organism
native enzyme 13.9fold to homogeneity by acetone precipitation, gel filtration, and anion exchange chromatography Balistes capriscus

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Balistes capriscus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.03
-
 purified enzyme, pH 10.5, 25°C Balistes capriscus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Balistes capriscus Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23200, SDS-PAGE Balistes capriscus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Balistes capriscus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 80% of maximal activitx at 20°C, maximal activity at 40°C, 50% at 55°C, profile, overview Balistes capriscus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 55 purified enzyme, 50% activity remaining at 55°C, stable at 20-40°C Balistes capriscus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.76
-
 Nalpha-benzoyl-DL-arginine 4-nitroanilide pH 10.5, 25°C Balistes capriscus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
 a highly alkaline trypsin from the Grey trigger fish Balistes capriscus

pH Range

pH Minimum pH Maximum Comment Organism
8 12 activity at pH 9.0, pH 11.5, and pH 12.0 is 86.5%, 92.6% and 52.4% of maximal activity at pH 10.5, respectively, profile, overview Balistes capriscus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 13 purified enzyme, completely stable at pH 7.0-12.0, profile, overview Balistes capriscus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40.6
-
 Nalpha-benzoyl-DL-arginine 4-nitroanilide pH 10.5, 25°C Balistes capriscus