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Literature summary for 3.4.21.4 extracted from

  • Villalba-Villalba, A.G.; Ramirez-Suarez, J.C.; Valenzuela-Soto, E.M.; Sanchez, G.G.; Ruiz, G.C.; Pacheco-Aguilar, R.
    Trypsin from viscera of vermiculated sailfin catfish, Pterygoplichthys disjunctivus, Weber, 1991: its purification and characterization (2013), Food Chem., 141, 940-945.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
benzamidine 80.2% inhibition Pterygoplichthys disjunctivus
N-alpha-p-tosyl-L-lysine chloromethyl ketone 85.4% inhibition Pterygoplichthys disjunctivus
NaCl trypsin activity decreases continuously as NaCl concentration increases from 0–30% Pterygoplichthys disjunctivus
phenyl methyl sulphonyl fluoride 100% inhibition Pterygoplichthys disjunctivus
Soybean trypsin inhibitor 75.6% inhibition Pterygoplichthys disjunctivus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 benzoyl-DL-Arg-4-nitroanilide pH 8.0, 25°C Pterygoplichthys disjunctivus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
 x * 27500, SDS-PAGE Pterygoplichthys disjunctivus

Organism

Organism UniProt Comment Textmining
Pterygoplichthys disjunctivus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pterygoplichthys disjunctivus

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
 Pterygoplichthys disjunctivus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.34
-
 pH 8.0, 25°C Pterygoplichthys disjunctivus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-DL-Arg-4-nitroanilide + H2O
-
 Pterygoplichthys disjunctivus benzoyl-DL-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 27500, SDS-PAGE Pterygoplichthys disjunctivus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pterygoplichthys disjunctivus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable below Pterygoplichthys disjunctivus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.46
-
 benzoyl-DL-Arg-4-nitroanilide pH 8.0, 25°C Pterygoplichthys disjunctivus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pterygoplichthys disjunctivus