Cloned (Comment) | Organism |
---|---|
wild-type enzyme,variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa, and variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa, expression in yeast | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
crystals of variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa, and variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa obtained in presence of benzamidine | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one | - |
Rattus norvegicus | |
benzamidine | - |
Rattus norvegicus | |
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester | - |
Rattus norvegicus | |
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Pefachrome tPA | native enzyme | Rattus norvegicus | |
0.0229 | - |
Pefachrome tPA | variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa | Rattus norvegicus | |
0.0472 | - |
Pefachrome tPA | variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Pefachrome tPA + H2O | - |
Rattus norvegicus | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000805 | - |
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester | native enzyme | Rattus norvegicus | |
0.0000247 | - |
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one | variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa | Rattus norvegicus | |
0.000054 | - |
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one | variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa | Rattus norvegicus | |
0.000122 | - |
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one | native enzyme | Rattus norvegicus | |
0.00152 | - |
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester | variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa | Rattus norvegicus | |
0.00605 | - |
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone | variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa | Rattus norvegicus | |
0.00651 | - |
benzamidine | native enzyme | Rattus norvegicus | |
0.00871 | - |
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester | variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa | Rattus norvegicus | |
0.0256 | - |
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone | native enzyme | Rattus norvegicus | |
0.0322 | - |
benzamidine | variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa | Rattus norvegicus | |
0.0445 | - |
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone | variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa | Rattus norvegicus | |
0.143 | - |
benzamidine | variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa | Rattus norvegicus |