Literature summary for 3.4.21.4 extracted from

Jiang, C.; Bao, R.; Chen, Y.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of Sagittaria sagittifolia arrowhead protease inhibitor API-A in complex with bovine trypsin (2008), Acta Crystallogr. Sect. F, 64, 1060-1062.

Search for more literature for 3.4.21.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Bos taurus

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with Sagittaria sagittifolia arrowhead protease inhibitor API-A, sitting drop vapour diffusion method, using 0.1 M sodium cacodylate pH 6.5, 0.2 M ammonium sulfate, and 20% PEG 8000	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
Sagittaria sagittifolia arrowhead protease inhibitor A	API-A	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-agarose affinity resin column chromatography and Superdex 200 gel filtration	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)