Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
liquid-liquid extraction with polyethyleneglycol/sodium citrate aqueous two-phase systems, aqueous two-phase systems formed by PEG3350 and NaCit pH 5.2 show the best separation capability, the addition of NaCl up to a final concentration of 7% (w/w) and the decrease of top/bottom volume ratio to 0.1 leads to the recovery of 60% of pancreatic trypsin in a concentrated form in the top phase with a 3fold purification | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O | - |
Bos taurus | N-alpha-benzoyl-L-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRP | - |
Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
pH 5.2 prevents autocatalytic hydrolysis of trypsin | Bos taurus |