Application | Comment | Organism |
---|---|---|
medicine | commercial-level production of trypsin in transgenic maize, the availability of this reagent should allow for the replacement of animal-derived trypsin in the processing of pharmaceutical proteins | Bos taurus |
Cloned (Comment) | Organism |
---|---|
commercial-level production of trypsin in transgenic maize, functionally equivalent to native bovine pancreatic trypsin | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
N-alpha-benzoyl-L-arginine 4-nitroanilide | pH 8.3, 25°C, recombinant enzyme from Zea may | Bos taurus | |
3.2 | - |
N-alpha-benzoyl-L-arginine 4-nitroanilide | pH 8.3, 25°C, native bovine trypsin | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23300 | - |
MALDI-TOF mass spectrometry | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant bovine trypsin from maize has glycoforms | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzoyl-L-arginine 4-nitroanilide + H2O | - |
Bos taurus | N-alpha-benzoyl-L-arginine + 4-nitroaniline | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
recombinant enzyme from Zea may | Bos taurus |
8.7 | - |
native bovine trypsin | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 10.2 | pH 6.9: about 35% of maximal activity, pH 10.2: about 40-50% of maximal activity, native and recombinant enzyme | Bos taurus |