Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.4 extracted from

  • Ben Khaled, H.; Jellouli, K.; Souissi, N.; Ghorbel, S.; Barkia, A.; Nasri, M.
    Purification and characterization of three trypsin isoforms from viscera of sardinelle (Sardinella aurita) (2011), Fish Physiol. Biochem., 37, 123-133.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Sardinella aurita
Co2+
-
 Sardinella aurita
Cu2+
-
 Sardinella aurita
EDTA a metalloenzyme inhibitor, 8% inhibition at 5 mM Sardinella aurita
Hg2+ strong inhibition Sardinella aurita
Mn2+
-
 Sardinella aurita
additional information no inhibition by DTNB and 2-mercaptoethanol Sardinella aurita
NaCl relative activities of trypsin isozymes A, B, and C at 15% NaCl are approximately 39%, 38% and 40% of maximal activity, respectively Sardinella aurita
phenylmethylsulfonyl fluoride a serine protease inhibitor, over 90% inhibition of trypsin isozymes A, B and C at 5 mM Sardinella aurita
Soybean trypsin inhibitor a serine protease inhibitor, complete inhibition of trypsin isozymes A, B and C at 1 mg/ml Sardinella aurita
Zn2+
-
 Sardinella aurita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide isozyme trypsin B, pH 9.0, 25°C Sardinella aurita
0.1
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide isozyme trypsin C, pH 9.0, 25°C Sardinella aurita
0.125
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide isozyme trypsin A, pH 9.0, 25°C Sardinella aurita

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+, Mg2+, K+, and Na+ do not affect the enzyme's proteolytic activity Sardinella aurita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28800
-
 x * 28800, trypsin isozymes A, B and C, SDS-PAGE Sardinella aurita

Organism

Organism UniProt Comment Textmining
Sardinella aurita
-
 trypsin isozymes A, B and C
-

Purification (Commentary)

Purification (Comment) Organism
native trypsin isozymes A, B and C 5.95fold, 9.26fold, and 15.93fold, respectively, from viscera by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Sardinella aurita

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
 Sardinella aurita
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.12
-
 purified native trypsin A, pH 9.0, 25°C Sardinella aurita
35.94
-
 purified native trypsin B, pH 9.0, 25°C Sardinella aurita
61.83
-
 purified native trypsin C, pH 9.0, 25°C Sardinella aurita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Sardinella aurita Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 28800, trypsin isozymes A, B and C, SDS-PAGE Sardinella aurita

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 trypsin isozyme B Sardinella aurita
55
-
 trypsin isozymes A and C Sardinella aurita

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 activity range of trypsin isozymes A, B and C, 20% of maximal activity at 20°C and 80°C, profiles, overview Sardinella aurita

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified trypsin isozymes A, B and C, 60 min, completely stable up to Sardinella aurita
50
-
 purified trypsin isozymes A, B and C, 60 min, 20% activity remaining Sardinella aurita
60
-
 purified trypsin isozymes A, B and C, 60 min, 15% activity remaining Sardinella aurita
70
-
 purified trypsin isozymes A, B and C, 60 min, inactivation Sardinella aurita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide isozyme trypsin B, pH 9.0, 25°C Sardinella aurita
2.24
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide isozyme trypsin A, pH 9.0, 25°C Sardinella aurita
5.76
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide isozyme trypsin C, pH 9.0, 25°C Sardinella aurita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 trypsin isozymes A, B and C Sardinella aurita

pH Range

pH Minimum pH Maximum Comment Organism
5 12 activity range of trypsin isozymes A, B and C, 10-20% of maximal activity at pH 5.0 and pH 12.0, profiles, overview Sardinella aurita

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12 purified trypsin isozymes A, B and C, completely stable at pH 8.0-10.0, 60% remaining activity at pH 12.0 and pH 7.0, 20% remaining activity at pH 6.0 Sardinella aurita