3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase This is an abbreviated version! For detailed information about alkylglycerophosphoethanolamine phosphodiesterase, go to the full flat file .
Reaction
1-alkyl-sn-glycero-3-phosphoethanolamine +
H2O =
1-alkyl-sn-glycerol 3-phosphate +
ethanolamine
Synonyms (ecto)nucleotide pyrophosphatase/phosphodiesterase 2 [(E)NPP2], ATX, ATX/lysoPLD, ATX/NPP2, AtxB, ATXgamma, autocrine motility factor, autotaxin, autotaxin (ATX), autotaxin beta, autotaxin gamma, autotaxin/lysoPLD, ecto-nucleotide pyrophosphatase/phosphodiesterase-2, ectonucleotide pyrophosphatase phosphodiesterase-2, ectonucleotide pyrophosphatase/phosphodiesterase, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP, ENPP2, GDE1, GDE4, GDE7, GDPD1, GDPD3, glycerophosphodiesterase, hATX, Heminecrolysin, human autotaxin, human autotaxin alpha, human autotaxin beta, LPLD, lyso-PLD, lysophospholipase D, lysophospholipase-D, lysoPLD, lysoPLD autotaxin, lysoPLD/NPP2, mATX, mATXgamma, More, NPP-2, NPP2, NPP2alpha, NPP2gamma, nucleotide phosphodiesterase/pyrophosphatase (NPP), nucleotide pyrophosphatase-phosphodiesterase, nucleotide pyrophosphatase/phosphodiesterase 2, PD-Ialpha, PD-Ialpha/ATX, phosphodiesterase Ialpha, phosphodiesterase Ialpha/autotoxin, phosphodiesterase, alkylglycerophosphorylethanolamine, PLD, rhATX S48, zATX
ECTree
Metals Ions
Metals Ions on EC 3.1.4.39 - alkylglycerophosphoethanolamine phosphodiesterase
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KSCN
-
200 mM, 37% increase of activity
Na3VO4
-
enzyme activity requires Na3VO4, not necessary after purification
NaCl
-
200 mM, 37% increase of activity
Ca2+
-
activates, dependent on
Ca2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Ca2+
similar stimulation of LysoPLD activity by addition of Ca2+ and Mg2+ occurs. Mg2+ or Ca2+ may act to stabilize the structure of ATX or shield the electric charge of the substrate
Ca2+
stimulated by micromolar concentrations of Ca2+
Ca2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Ca2+
stimulated by micromolar concentrations of Ca2+
Ca2+
-
5 mM required for activity
Co2+
-
lysoPLD activity is increased when Co2+ is added. Addition of Zn2+, Mn2+, Ni2+, or Co2+ to diluted egg white alters preference patterns of lysoPLD toward choline-containing substrates
Co2+
activity of hATX S48 protein is significantly stimulated by addition of Co2+
Co2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Co2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Co2+
-
extracellular enzyme
Co2+
-
purified enzyme is incubated with Co2+. LysoPLD activity in serum is more sensitive to Co2+
Mg2+
-
required
Mg2+
-
activates, dependent on
Mg2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Mg2+
similar stimulation of LysoPLD activity by addition of Ca2+ and Mg2+ occurs. Mg2+ or Ca2+ may act to stabilize the structure of ATX or shield the electric charge of the substrate
Mg2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Mg2+
-
low effect in rabbit kidney
Mg2+
-
the purified enzyme is incubated with Mg2+. Purified enzyme is more sensitive to Mg2+ than to Co2+. Enzyme activity requires Mg2+
Mn2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Mn2+
-
required for cell surface/intergrin binding
Mn2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Mn2+
-
extracellular enzyme
Mn2+
-
the purified enzyme is incubated with Mn2+
Ni2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Ni2+
Mg2+-ATP treated sample is applied to the Ni2+ charged chelating sepharose. Most effective affinity chelating metal
Ni2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Ni2+
-
extracellular enzyme
Zn2+
-
enzyme-bound, required
Zn2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Zn2+
-
two catalytic zinc ions in the active site
Zn2+
check the sensitivity to metals for the human and murine isoforms, concentrations ranging from 0.1 to 1000 mM
Zn2+
enzyme-bound, required
Zn2+
-
extracellular enzyme
Zn2+
required, active site bound
additional information
-
the catalytic domain shows a characteristic bimetallic active site
additional information
Loxosceles sp.
-
the catalytic domain shows a characteristic bimetallic active site
additional information
-
the catalytic domain shows a characteristic bimetallic active site
additional information
-
no effect on enzyme activity by Ba2+, Mg2+, Ca2+, and Ni2+
additional information
-
the catalytic domain shows a characteristic bimetallic active site