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3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase

This is an abbreviated version!
For detailed information about alkylglycerophosphoethanolamine phosphodiesterase, go to the full flat file.

Word Map on EC 3.1.4.39

Reaction

1-alkyl-sn-glycero-3-phosphoethanolamine
+
H2O
=
1-alkyl-sn-glycerol 3-phosphate
 +
ethanolamine

Synonyms

(ecto)nucleotide pyrophosphatase/phosphodiesterase 2 [(E)NPP2], ATX, ATX/lysoPLD, ATX/NPP2, AtxB, ATXgamma, autocrine motility factor, autotaxin, autotaxin (ATX), autotaxin beta, autotaxin gamma, autotaxin/lysoPLD, ecto-nucleotide pyrophosphatase/phosphodiesterase-2, ectonucleotide pyrophosphatase phosphodiesterase-2, ectonucleotide pyrophosphatase/phosphodiesterase, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP, ENPP2, GDE1, GDE4, GDE7, GDPD1, GDPD3, glycerophosphodiesterase, hATX, Heminecrolysin, human autotaxin, human autotaxin alpha, human autotaxin beta, LPLD, lyso-PLD, lysophospholipase D, lysophospholipase-D, lysoPLD, lysoPLD autotaxin, lysoPLD/NPP2, mATX, mATXgamma, More, NPP-2, NPP2, NPP2alpha, NPP2gamma, nucleotide phosphodiesterase/pyrophosphatase (NPP), nucleotide pyrophosphatase-phosphodiesterase, nucleotide pyrophosphatase/phosphodiesterase 2, PD-Ialpha, PD-Ialpha/ATX, phosphodiesterase Ialpha, phosphodiesterase Ialpha/autotoxin, phosphodiesterase, alkylglycerophosphorylethanolamine, PLD, rhATX S48, zATX

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.39 alkylglycerophosphoethanolamine phosphodiesterase

Cloned

Cloned on EC 3.1.4.39 - alkylglycerophosphoethanolamine phosphodiesterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha isoform of ATX, overexpression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
atx-/-, atx+/-, and atx+/+ uncover the physiological role of ATX and to identify the endogenous product of ATX, ATX gene is targeted with an ATXgene-targeting vector. Plasma lysoPLD activity, plasma lysophosphatidic acid concentrations and plasma ATX protein level in atx+/-mice are about half the values found in atx+/+ mice. Embryos, early blood vessels appear to form properly, but fail to develop into mature vessels. ATX-null mice are lethal around embryonic day 10.5. Despite the reduced LPA level, heterozygous mice appear phenotypically normal. Viability and fecundity are similar to those in wild-type mice. Morphologies of atx+/+ and atx-/-embryo proper at E8.5, E9.5, and E10.5. Defects in vascular remodeling in atx-/-embryos shown. ATX and LPA stabilize preformed vessels and prevent endothelial disassembly in allantois explants
-
cloned from 129S6/Sv mouse brain, expression in COS-7 cells
-
co-transfection of MBEC4 cells with 2 plasmids, mlysoPLDpCAGGS26 and pSV2-neo, screening by Western blotting and immunocytochemistry with an anti-ATX mAb (4F1)14 and by measuring the lysoPLD activities of the culture media
-
COS-7 cells (ATCC: CRL-1651) and HEK 293 cells. To gain further insight into the possible functional role of ATX in the brain, analysis of its cellular expression in the brain under physiological conditions and following trauma. Preparation and purification of oligodendrocyte precursor cells: Primary mixed glial cultures are prepared from the cerebral hemispheres of P0–P2 Sprague-Dawley rat pups from which the meninges has been stripped
-
expressed in B-103 cells
-
expressed in COS-7 cells
-
expressed in COS-7 cells (ATCC: CRL-1651) and HEK 293 cells. Compared distribution of overexpressed ATX in COS-7 and HEK293 cells
-
expressed in Escherichia coli BL21 and Escherichia coli TOP10 strain. Construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX
expressed in HEK-293 cells
-
expressed in HEK-293T cells
-
expressed in Mus musculus
-
expressed in three different hosts, Sf9 insect cells and CHO cells, expressed in COS cells. ATXalpha, human, 915 amino acids, lacks exon 21, this particular isoform expresses the intron 12 in which a cleavage site is present, leading to a rapid catabolism of the isoform. ATXbeta, human, 863 amino acids, lacks exons 12 and 21. Cloning of the three isoforms alpha, beta, and gamma
expressed in two different hosts, Sf9 insect cells and chinese hamster ovary cells, expressed in COS cells, cloning of the three isoforms alpha, beta, and gamma
expressed in two different hosts, Sf9 insect cells and CHO cells, expressed in COS cells
expressed in two different hosts, Sf9 insect cells and CHO cells, expressed in COS cells, cloning of the three isoforms alpha, beta, and gamma
expression in Bac-to-Bac baculovirus in Sf9 insect cells
-
expression in Escherichia coli DH5alpha
-
expression in HEK-293 cell
expression in Sf9 cell
functional expression of the ATX-Fc fusion protein in HEK-293 cells with secretion of the enzyme to the medium
-
functional overexpression of V5-tagged ATX and secretion into conditioned medium
-
gene atx, DNA and amino acid sequence determination and analysis, recombinant expression in HEK-293 cells
-
generate mouse overexpressing circulating autotaxin: amplification of human 1-antitrypsin (A1AT) promoter by PCR (from a Yac clone obtained from ATCC), injection of DNA into pronuclei of 1-cellstage FVB mouse embryos, transfer of injected cells into the oviduct of pseudopregnant female mouse. Transfection of CHO cells with a vector for expression of a fusion protein containing the human immunoglobulin-kappa chain leader sequence, a His-tag, and a cDNA-sequence encoding aminoacids 52-915 of human autotaxin-gamma
-
human ATX cDNA cloned with a C-terminal His-tag in pcDNA3 for transfection of HEK293T cells
-
isolation of cDNA encoding ATX from HEC cDNA library and transfection of COS-7 African green monkey kidney cells, recombinant expression and purification and immunoblot analysis for showing that HECs synthesize and secrete ATX
-
real-time quantitative PCR expression analysis
recombinant expression of GDE1 in COS-7 cells
-
stable expression of ATX in HEK-293 cells with secretion of the recombinnat enzyme
transfection of FTC-238 cells with pEGFP-C3/ATX-construct for secretion of expressed ATX and with pD38tm/ATX-construct for production of membrane-anchored ATX respectively. sATX clones show higher cell motility than mATX clones (transwell filter assay), addition of interleukin-1beta to culture medium reduces cell motility due to downregulating ATX gene activity (mRNA expression level analysed by quantitative-RT-PCR), colony soft agar assay of sATX-clones results in bigger amount and bigger diameter of colonies compared to mATX-clones