3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase
This is an abbreviated version!
For detailed information about alkylglycerophosphoethanolamine phosphodiesterase, go to the full flat file.
Word Map on EC 3.1.4.39
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3.1.4.39
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lysophosphatidic
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lysophosphatidylcholine
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phospholipid
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fibrosis
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lysophospholipids
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atx-lpa
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metastasis
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choline
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protein-coupled
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melanoma
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autocrine
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motility-stimulating
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ectoenzyme
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lpa-producing
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sphingosine
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analysis
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pruritus
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drug development
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ectonucleoside
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lpars
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e-npps
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motogenic
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lpa-induced
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medicine
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molecular biology
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lysophosphatidylserine
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ntpdases
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diagnostics
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diphosphohydrolase
- 3.1.4.39
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lysophosphatidic
- lysophosphatidylcholine
- phospholipid
- fibrosis
- lysophospholipids
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atx-lpa
- metastasis
- choline
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protein-coupled
- melanoma
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autocrine
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motility-stimulating
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ectoenzyme
-
lpa-producing
- sphingosine
- analysis
- pruritus
- drug development
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ectonucleoside
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lpars
- e-npps
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motogenic
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lpa-induced
- medicine
- molecular biology
- lysophosphatidylserine
- ntpdases
- diagnostics
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diphosphohydrolase
Reaction
Synonyms
(ecto)nucleotide pyrophosphatase/phosphodiesterase 2 [(E)NPP2], ATX, ATX/lysoPLD, ATX/NPP2, AtxB, ATXgamma, autocrine motility factor, autotaxin, autotaxin (ATX), autotaxin beta, autotaxin gamma, autotaxin/lysoPLD, ecto-nucleotide pyrophosphatase/phosphodiesterase-2, ectonucleotide pyrophosphatase phosphodiesterase-2, ectonucleotide pyrophosphatase/phosphodiesterase, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP, ENPP2, GDE1, GDE4, GDE7, GDPD1, GDPD3, glycerophosphodiesterase, hATX, Heminecrolysin, human autotaxin, human autotaxin alpha, human autotaxin beta, LPLD, lyso-PLD, lysophospholipase D, lysophospholipase-D, lysoPLD, lysoPLD autotaxin, lysoPLD/NPP2, mATX, mATXgamma, More, NPP-2, NPP2, NPP2alpha, NPP2gamma, nucleotide phosphodiesterase/pyrophosphatase (NPP), nucleotide pyrophosphatase-phosphodiesterase, nucleotide pyrophosphatase/phosphodiesterase 2, PD-Ialpha, PD-Ialpha/ATX, phosphodiesterase Ialpha, phosphodiesterase Ialpha/autotoxin, phosphodiesterase, alkylglycerophosphorylethanolamine, PLD, rhATX S48, zATX
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3.1.4.39 alkylglycerophosphoethanolamine phosphodiesteraseAdvanced search results
results (
results (KM Value
KM Value on EC 3.1.4.39 - alkylglycerophosphoethanolamine phosphodiesterase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.1
1-alkyl-sn-glycero-3-phosphoethanolamine
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achieved under optimal assay conditions, almost same results in blood plasma
0.0083
1-linoleoyl-2-lyso-glycerophoshorylcholine
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Vmax is 0.019 micromol/min/mg
0.32
1-O-alkyl-sn-glycero-3-phosphocholine
pH 7.4, temperature not specified in the publication
0.0267
1-O-hexadecyl-2-lyso-glycerophosphorylcholine
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Vmax is 0.29 micromol/min/mg, lysoPLD activity is more than twice that observed with 1-palmitoyl-glycerophoshorylcholine
0.0207
1-palmitoyl-2-lyso-glycerophoshorylcholine
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Vmax is 0.059 micromol/min/mg
0.176
1-palmitoyl-glycerophoshorylcholine
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Vmax is 0.3 micromol/min/mg, best substrate
0.101
1-stearoyl-2-lyso-glycerophoshorylcholine
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Vmax is 0.035 micromol/min/mg
0.0063
5-[[(23R)-33-(4-[[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,33-dioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
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0.004
coumarin-phosphate-fluorescein
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coumarin-phosphate-fluorescein is a fluorescence resonance energy transfer (FRET)-based sensor of phosphodiesterase activity. Very useful substrate for enzymatic analysis of purified autotaxin/lysoPLD and can also be used to detect autotaxin/lysoPLD activity in serum-free conditioned culture medium isolated from cells expressing the enzyme
0.004
[5-[[(23S)-33-(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,26,33-trioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]acetic acid
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[5-[[(23S)-33-(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,26,33-trioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]acetic acid can be used to detect autotaxin/lysoPLD activity in human and mouse blood
0.7
4-nitrophenyl-TMP
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wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
4.8
4-nitrophenyl-TMP
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mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.0026
FS-3
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wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.019
FS-3
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mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.1
lysophosphatidylcholine
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kinetic analysis of recombinant autotaxin/lysoPLD using [14C] lysophosphatidylcholine substrate
0.47
lysophosphatidylcholine
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in presence of 0.25 mM Zn2+ plus 10 mM L-histidine
1
lysophosphatidylcholine
pH 7.4, temperature not specified in the publication
0.066
lysophosphatidylcholine 16:0
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wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.094
lysophosphatidylcholine 16:0
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mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.12
lysophosphatidylcholine 16:0
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mutant enzyme H434Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.126
lysophosphatidylcholine 16:0
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mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.089
lysophosphatidylcholine 18:0
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wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.115
lysophosphatidylcholine 18:0
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mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.27
lysophosphatidylcholine 18:0
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mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.069
lysophosphatidylcholine 18:1
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mutant enzyme H420Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.073
lysophosphatidylcholine 18:1
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wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.105
lysophosphatidylcholine 18:1
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mutant enzyme H434Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
0.126
lysophosphatidylcholine 18:1
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mutant enzyme H226Q, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C
additional information
additional information
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suggestion that lysoPLD predominantly utilizes saturated forms of lysophosphatidylcholine
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additional information
additional information
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affinity for lysophosphatidylcholine is significantly higher than for nucleotides
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additional information
additional information
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capable of hydrolyzing sphingosylphosphorylcholine with a significantly higher km-value, also able to hydrolyze nucleotide triphosphates di-adenosine polyphosphates and artificial phosphodiester substrates such as p-nitrophenyl thymidine phosphate with Km-values close to 1 and optimal activity observed at alkaline pH
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additional information
additional information
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steady-state, single-turnover, and transient kinetics, kinetic analysis and modeling, using artificial fluorescent substrate FS-3, overview
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