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3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase

This is an abbreviated version!
For detailed information about alkylglycerophosphoethanolamine phosphodiesterase, go to the full flat file.

Word Map on EC 3.1.4.39

Reaction

1-alkyl-sn-glycero-3-phosphoethanolamine
+
H2O
=
1-alkyl-sn-glycerol 3-phosphate
 +
ethanolamine

Synonyms

(ecto)nucleotide pyrophosphatase/phosphodiesterase 2 [(E)NPP2], ATX, ATX/lysoPLD, ATX/NPP2, AtxB, ATXgamma, autocrine motility factor, autotaxin, autotaxin (ATX), autotaxin beta, autotaxin gamma, autotaxin/lysoPLD, ecto-nucleotide pyrophosphatase/phosphodiesterase-2, ectonucleotide pyrophosphatase phosphodiesterase-2, ectonucleotide pyrophosphatase/phosphodiesterase, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP, ENPP2, GDE1, GDE4, GDE7, GDPD1, GDPD3, glycerophosphodiesterase, hATX, Heminecrolysin, human autotaxin, human autotaxin alpha, human autotaxin beta, LPLD, lyso-PLD, lysophospholipase D, lysophospholipase-D, lysoPLD, lysoPLD autotaxin, lysoPLD/NPP2, mATX, mATXgamma, More, NPP-2, NPP2, NPP2alpha, NPP2gamma, nucleotide phosphodiesterase/pyrophosphatase (NPP), nucleotide pyrophosphatase-phosphodiesterase, nucleotide pyrophosphatase/phosphodiesterase 2, PD-Ialpha, PD-Ialpha/ATX, phosphodiesterase Ialpha, phosphodiesterase Ialpha/autotoxin, phosphodiesterase, alkylglycerophosphorylethanolamine, PLD, rhATX S48, zATX

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.39 alkylglycerophosphoethanolamine phosphodiesterase

Purification

Purification on EC 3.1.4.39 - alkylglycerophosphoethanolamine phosphodiesterase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from CHO cells: sequential metal ion affinity and gel filtration chromatography, exchanged into phosphate-buffered saline and concentrated to 1 mg/ml of monomeric autotaxin by centrifugal filtration
-
from fetal bovine serum. SDS-PAGE, protein detection by silver staining
-
gel filtration, SDS–PAGE. Establish purification protocol for recombinant hATX S48 protein. Introduction of the construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX
includes a protease inhibitor at all steps to avoid isolation of the products of proteolysis. Purified to apparent homogeneity from rat brain nuclear fractions using 1-[14C]palmitoyl-glycerophosphorylcholine as a substrate
-
native enzyme from brain nuclear fractions by anion exchange chromatography, gel filtration, and again anion exchange chromatography
-
prepared from a Vaccinia viral lysate through the concanavalin A-agarose step
-
recombinant ATX from HEK-293 cell culture supernatant
recombinant ATX is purified using TALON resin beads
-
recombinant ATX-Fc fusion protein from HEK-293 cells cell medium by affinity chromatography with protein A sepharose followed by cleavage with thrombin. Detagged ATX protein is further purified to essential homogeneity by gel filtration
-
recombinant His-tagged ATX alpha isoform from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration
-
two recombinant mouse ATX proteins (wild-type ATX and catalytically inactive T209A mutant ATX) with a His tag at the N-terminus are expressed and purified using a baculovirus system and nickel column chromatography
-
using GSH-Sepharose
-
Western blot in cell homogenates requires a pre-purification with concanavalin A-sepharose
-
with and without His-tag
-