Cloned (Comment) | Organism |
---|---|
generate mouse overexpressing circulating autotaxin: amplification of human 1-antitrypsin (A1AT) promoter by PCR (from a Yac clone obtained from ATCC), injection of DNA into pronuclei of 1-cellstage FVB mouse embryos, transfer of injected cells into the oviduct of pseudopregnant female mouse. Transfection of CHO cells with a vector for expression of a fusion protein containing the human immunoglobulin-kappa chain leader sequence, a His-tag, and a cDNA-sequence encoding aminoacids 52-915 of human autotaxin-gamma | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lysophosphatic acid | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
immunoblotting, 3D1 antibody (1/500), a polyclonal antibody raised against the peptide sequence in autotaxin CKKPDQHFKPYMKQHLPKRL and a polyclonal antibody to amino acids 541-720 of autotoxin | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | Homo sapiens | - |
lysophosphatidic acid + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
from CHO cells: sequential metal ion affinity and gel filtration chromatography, exchanged into phosphate-buffered saline and concentrated to 1 mg/ml of monomeric autotaxin by centrifugal filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | - |
Homo sapiens | lysophosphatidic acid + choline | - |
? | |
lysophosphatidylcholine + H2O | radiolabeled substrate and fluorogenic substrate FS-3 | Homo sapiens | lysophosphatidic acid + choline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
autotaxin | - |
Homo sapiens |
ENPP2 | - |
Homo sapiens |
lysoPLD | - |
Homo sapiens |