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Literature summary for 3.1.4.39 extracted from

  • Haga, A.; Hashimoto, K.; Tanaka, N.; Nakamura, K.T.; Deyashiki, Y.
    Scalable purification and characterization of the extracellular domain of human autotaxin from prokaryotic cells (2008), Protein Expr. Purif., 59, 9-17.
    View publication on PubMed

Application

Application Comment Organism
drug development ATX is one of the target molecules for cancer therapy, therefore the immuno-neutralization by an antibody which is able to recognize the catalytic site, or the low molecular weight chemical inhibitors based on structural biology should be a useful tool for prevention or treatment of tumor progression and metastasis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 and Escherichia coli TOP10 strain. Construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information rhATX S48 site-directed mutagenesis, recombinant human ATX, creation of a full-length recombinant molecule by using prokaryotic cells. Shows lysoPLD enzymatic activity. hATX S48 Ser-48-C terminal of human ATX-T (L46720) cDNA is amplified. hATX S48 protein shows both lysoPLD and PDE activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane N-terminal transmembrane type glycoprotein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ similar stimulation of LysoPLD activity by addition of Ca2+ and Mg2+ occurs. Mg2+ or Ca2+ may act to stabilize the structure of ATX or shield the electric charge of the substrate Homo sapiens
Co2+ activity of hATX S48 protein is significantly stimulated by addition of Co2+ Homo sapiens
Mg2+ similar stimulation of LysoPLD activity by addition of Ca2+ and Mg2+ occurs. Mg2+ or Ca2+ may act to stabilize the structure of ATX or shield the electric charge of the substrate Homo sapiens
Ni2+ Mg2+-ATP treated sample is applied to the Ni2+ charged chelating sepharose. Most effective affinity chelating metal Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
transmembrane protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lysophosphatidyl choline + H2O Homo sapiens
-
lysophosphatidic acid + choline
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13822
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS–PAGE. Establish purification protocol for recombinant hATX S48 protein. Introduction of the construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HT-1080 cell Various concentrations of hATX S48 protein are exposed to HT-1080 cells and motile area is analyzed. hATX S48 protein also affects cell proliferation Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
biochemical and cell biological activity of hATX S48 protein measured Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidyl choline + H2O
-
Homo sapiens lysophosphatidic acid + choline
-
?
lysophosphatidyl choline + H2O various concentration of lysophosphatidyl choline with or without 2.5 pM of hATX S48 are exposed to HT-1080 cells and motile area is analyzed Homo sapiens lysophosphatidic acid + choline
-
?
additional information Catalytic ability of ATX for p-nitrophenyl thymidine 5'-monophosphate and adenosine triphosphate hydrolysis are low compared with nucleotide pyrophosphatases-1/phosphodiesterases Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ATX one of the well-known tumor malignant factors Homo sapiens
autotaxin
-
Homo sapiens
human autotaxin
-
Homo sapiens
lysophospholipase D
-
Homo sapiens
lysoPLD
-
Homo sapiens
rhATX S48 recombinant human ATX (rhATX S48) from prokaryotic cells Homo sapiens