1.2.1.12: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) This is an abbreviated version! For detailed information about glyceraldehyde-3-phosphate dehydrogenase (phosphorylating), go to the full flat file .
Reaction
D-glyceraldehyde 3-phosphate +
phosphate +
NAD+ =
3-phospho-D-glyceroyl phosphate +
NADH +
H+
Synonyms 3-phosphoglyceraldehyde dehydrogenase, A4-GAPDH, A4-glyceraldehyde-3-phosphate dehydrogenase, AB-GAPDH, AnBn-GAPDH, AsGAPDH, At3g04120, BARS-38, CbbG, CgGAP, Clo1313_2095, complement-C3-binding protein, CP 17/CP 18, Ctherm_Gapdh, cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase, cytosolic phosphorylating glyceraldehyde-3-phosphate dehydrogenase, D-glyceraldehyde-3-phosphate dehydrogenase, D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), dehydrogenase, glyceraldehyde phosphate, dihydrogenase, glyceraldehyde phosphate, EcGAPDH, EcGAPDH1, FgGAPDH, FhGAPDH, G3PD, G3PDH, Ga3P dehydrogenase, Ga3PDHase, GADPH, GAP, GAP1, gap2, GapA, GapB, GAPC, GapC-1, GapC1, GapC2, GAPCp, GAPCp1, GAPCp2, GAPD, GAPDH, GAPDH type 1, GAPDH1, GAPDH2, GAPDH3, GAPDHS, GAPDS, GAPN, GBS GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase-S, glyceraldehyde phosphate dehydrogenase (NAD), glyceraldehyde-3 phosphate dehydrogenase, glyceraldehyde-3-P-dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NAD), glyceraldehyde-3-phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase, type I, glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein, glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS, glyceraldehyde-3-phosphate dehydrogenases, GPD, GPD2, Gra3PDH, GraP-DH, H.c-C3BP, hGAPDH, HsGAPDH, kmGAPDH1p, Larval antigen OVB95, Major larval surface antigen, Mtb-GAPDH, NAD+-dependent GAPDH, NAD+-dependent glyceraldehyde 3-phosphate dehydrogenase, NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD+-G-3-P dehydrogenase, NAD+-GAPDH, NAD-dependent Ga3PDHase, NAD-dependent glyceraldehyde 3-phosphate dehydrogenase, NAD-dependent glyceraldehyde phosphate dehydrogenase, NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase, NAD-G3PDH, NAD-GAPDH, NADH-glyceraldehyde phosphate dehydrogenase, P-37, p-GAPDH, PfGAPDH, phosphoglyceraldehyde dehydrogenase, phosphorylating NAD+-dependent GAPDH, Plasmin receptor, Plasminogen-binding protein, plastidial glyceraldehyde-3-phosphate dehydrogenase, pmGAPDH, PyGapdh, rmGAPDH, Rv1436, somatic GAPD, somatic glyceraldehyde 3-phosphate dehydrogenase, sperm-specific GAPDS, sperm-specific glyceraldehyde 3-phosphate dehydrogenase, sperm-specific glyceraldehyde-3-phosphate dehydrogenase, TaeNAD-GAPDH, TagapC, TDH1, TDH2, TDH3, TLAb, triose phosphate dehydrogenase, UDG, uracil-DNA glycosylase, vGPD
ECTree
Molecular Weight
Molecular Weight on EC 1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
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113000
recombinant enzyme, gel filtration
128000
-
equilibrium sedimentation
133000
-
enzyme form E9.0, gel filtration
138600
-
sucrose density gradient centrifugation
14000
-
x * 14000 + x * 37000, SDS-PAGE
140500
-
equilibrium sedimentation
145000 - 148000
-
equilibrium sedimentation, velocity sedimentation
146000
-
non-denaturing PAGE
146800
-
equilibrium sedimentation
148700
-
equilibrium sedimentation
161984
recombinant N-terminally prolongated enzyme, mass spectrometry
208000
-
native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS
209200
-
calculated from amino acid sequence
290000
-
gel filtration, pentalenolactone-insensitive enzyme
29500
-
4 * 29500, enzyme form E8.5, SDS-PAGE
315000
-
non-denaturing PAGE
33000
-
4 * 33000, enzyme form E9.5, SDS-PAGE
33700
-
4 * 33700, SDS-PAGE
35500
-
x * 35500 + x * 37000, SDS-PAGE
35673
x * 35673, calculated
35914
x * 35914, calculated from sequence
35960
x * 35960, calculated
35990
x * 35990, calculated
36100
x * 36100, calculated
36140
-
theoretical mass of the A subunit
36200
calculated from amino acid sequence
36500
-
4 * 36500, non-denaturing PAGE
36830
4 * 36830, MALDI-TOF mass spectrometry
37500
x * 37600, calculated, x * 37500, SDS-PAGE
37672
4 * 37672, MALDI-TOF mass spectrometry
39000
determined by SDS-PAGE and Western Blot analysis
39360
-
theoretical mass of the B subunit
40270
x * 40270, calculated
50000
-
4 * 50000, SDS-PAGE
51000
-
4 * 51000, native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS
61000
-
1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE
70000
-
SDS-PAGE, treatment of GAPDH with (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide at 0.01 mM for 10 min at 37°C results in formation of three bands (66, 68, and 76 kDa) corresponding roughly to a dimer of 70000 Da
75000
recombinant enzyme, gel filtration
98000
-
enzyme form E6.6, gel filtration
120000
-
enzyme form E8.5, gel filtration
120000
-
tetramer, determined by gel filtration
130000
-
tetramer, determined by gel filtration
130000
-
tetramer, estimated by gel filtration
140000 - 150000
-
sucrose density gradient centrifugation
140000 - 150000
native PAGE
142000
-
-
142000
-
equilibrium sedimentation
142000
-
low speed equilibrium sedimentation
142000
-
enzyme form E6.8, gel filtration
144000
-
gel filtration
144000
-
tetramer, calculated
145000
-
gel filtration
145000
-
velocity sedimentation
145000
recombinant His-tagged enzyme, gel filtration
148000
-
equilibrium sedimentation
148000
-
equilibrium sedimentation
150000
-
-
150000
N-terminally truncated enzyme after trypsin-treatment, Blue Native PAGE
150000
recombinant N-terminally prolongated enzyme, gel filtration
160000
-
gel filtration
160000
recombinant tagged enzyme, gel filtration
180000
-
-
180000
-
gel filtration, pentalenolactone-sensitive enzyme
33500
-
4 * 33500, SDS-PAGE
33500
-
4 * 33500, cytosolic enzyme, SDS-PAGE
35000
-
4 * 35000, SDS-PAGE
35000
-
4 * 35000, SDS-PAGE
35000
-
4 * 35000, SDS-PAGE
35000
-
x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol
36000
-
-
36000
-
subunit, SDS-PAGE
36000
-
subunit, SDS-PAGE
36000
-
SDS-PAGE, native enzyme
36000
SDS-PAGE, native enzyme
36000
-
monomer, determined by SDS-PAGE
36000
-
monomer, determined by SDS-PAGE
36000
-
x * 36000, SDS-PAGE
36000
-
x * 36000, SDS-PAGE
36000
-
4 * 36000, SDS-PAGE
36000
-
4 * 36000, SDS-PAGE
36000
-
4 * 36000, SDS-PAGE
36000
-
4 * 36000, SDS-PAGE
36000
-
4 * 36000, SDS-PAGE
36000
-
4 * 36000, SDS-PAGE
36000
-
x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE
36446
x * 36446, calculated from amino acid sequence
36446
x * 36446, calculated, x * 38000, SDS-PAGE
37000
-
-
37000
-
endogenously expressed in COS7L cells, immunoblot
37000
ADP-ribosylated enzyme subunit, SDS-PAGE
37000
-
4 * 37000, SDS-PAGE
37000
-
4 * 37000, SDS-PAGE
37000
-
4 * 37000, SDS-PAGE
37000
-
4 * 37000, SDS-PAGE
37000
-
4 * 37000, SDS-PAGE
37000
4 * 37000, SDS-PAGE
37000
4 * 37000, SDS-PAGE
37000
-
x * 37000, SDS-PAGE
37000
-
x * 37000, SDS-PAGE
37000
-
x * 14000 + x * 37000, SDS-PAGE
37000
-
x * 35500 + x * 37000, SDS-PAGE
37000
-
4 * 37000, denaturing SDS-PAGE
37600
-
x * 37600, calculated and SDS-PAGE
37600
x * 37600, calculated, x * 37500, SDS-PAGE
38000
native PAGE
38000
-
determined by SDS-PAGE and Western Blot analysis
38000
-
x * 38000, SDS-PAGE
38000
x * 38000, SDS-PAGE
38000
-
4 * 38000, SDS-PAGE
38000
4 * 38000, SDS-PAGE
38000
-
4 * 38000, GAPDH I and GAPDH II, SDS-PAGE
38000
-
4 * 38000, glyoxysomal enzyme, SDS-PAGE
38000
-
4 * 38000, glyoxysomal enzyme, SDS-PAGE
38000
-
x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE
38000
-
4 * 38000, cetyltrimethyl ammonium bromide PAGE
38000
x * 36446, calculated, x * 38000, SDS-PAGE
38500
-
4 * 38500, SDS-PAGE
38500
-
4 * 38500, non-denaturing PAGE
40000
-
SDS-PAGE
40000
-
4 * 40000, SDS-PAGE
40000
x * 40000, SDS-PAGE
40000
x * 40000, SDS-PAGE
40000
Q81X74, YP_027084
x * 40000, SDS-PAGE
40000
4 * 40000, N-terminally truncated enzyme after trypsin treatment, SDS-PAGE
41000
4 * 41000, SDS-PAGE
41000
-
x * 41000, SDS-PAGE
43000
-
4 * 43000, pentalenolactone-sensitive enzyme, gel filtration
43000
-
8 * 43000, pentalenolactone-insensitive enzyme, gel filtration
58000
full length subunit
58000
-
1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE
additional information
-
the enzyme possesses precise 222 symmetry. Pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. Three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer
additional information
-
Cys content is higher than that found in analogous enzymes
additional information
-
high content of beta-structure, 45%
additional information
-
comparison of structural properties
additional information
-
comparison of structural properties
additional information
-
comparison of structural properties
additional information
-
comparison of structural properties
additional information
Psithyrus suckleyi
-
comparison of structural properties
additional information
-
unusually low His content compared to other mammalian muscle acetaldehyde phosphate dehydrogenases