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Literature summary for 1.2.1.12 extracted from

  • Mountassif, D.; Baibai, T.; Fourrat, L.; Moutaouakkil, A.; Iddar, A.; El Kebbaj, M.S.; Soukri, A.
    Immunoaffinity purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from human erythrocytes (2009), Acta Biochim. Biophys. Sin. (Shanghai), 41, 399-406.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0178
-
NAD+ pH 8.5, 25°C Homo sapiens
0.0207
-
D-glyceraldehyde 3-phosphate pH 8.5, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, SDS-PAGE Homo sapiens
150000
-
PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
use of immunoaffinity column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
pH 8.5, 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ absolute specificity for NAD+ Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ absolute specificity for NAD+ Homo sapiens
NADH
-
Homo sapiens