Cloned (Comment) | Organism |
---|---|
gene CgGAP or GPD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, promoter analysis, six stress-response elements were found in the upstream region of the CgGAP gene. Recombinant expression in enzyme-deficient Saccharomyces cerevisiae from plasmid pYX212-CgGAP and functional complementation restoring the wild-type phenotype. The GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cerevisiae cells grown on glucose, determined via GFP-tagged gene expression analysis | Pichia kudriavzevii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
- |
Pichia kudriavzevii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Pichia kudriavzevii | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Pichia kudriavzevii WL2002-5 | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pichia kudriavzevii | - |
- |
- |
Pichia kudriavzevii WL2002-5 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pichia kudriavzevii | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pichia kudriavzevii WL2002-5 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, about, sequence determination and analysis | Pichia kudriavzevii |
More | the CgGAP protein consists of an N-terminal NAD+-binding domain and a central catalytic domain | Pichia kudriavzevii |
Synonyms | Comment | Organism |
---|---|---|
CgGAP | - |
Pichia kudriavzevii |
GAPDH | - |
Pichia kudriavzevii |
NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Pichia kudriavzevii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pichia kudriavzevii |
Organism | Comment | Expression |
---|---|---|
Pichia kudriavzevii | six stress-response elements were found in the upstream region of the CgGAP gene | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme of glycolysis, catalyses the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as the co-enzyme | Pichia kudriavzevii |