Crystallization (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
5.0 M guanidine-HCl, rapid and irreversible inactivation at 30°C | Geobacillus stearothermophilus |
8.0 M urea, unusually resistant at 30°C, 40°C and 50°C. Rapid inactivation at 55°C and at 60°C | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Geobacillus stearothermophilus | |
p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Geobacillus stearothermophilus |
148700 | - |
equilibrium sedimentation | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, in 67% saturated ammonium sulfate solution containing EDTA and dithiothreitol, stable for months | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl arsenate + NADH | - |
ir | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
10 min, about 10% inactivation | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme contains 4 equivalents of firmly bound NAD+ | Geobacillus stearothermophilus |