Information on EC 1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
1.2.1.12
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RECOMMENDED NAME
GeneOntology No.
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
Phosphorylation
redox reaction
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-
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reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Bifidobacterium shunt
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Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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Carbon fixation in photosynthetic organisms
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formaldehyde assimilation III (dihydroxyacetone cycle)
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gluconeogenesis I
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gluconeogenesis III
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glycerol degradation to butanol
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Glycolysis / Gluconeogenesis
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glycolysis I (from glucose 6-phosphate)
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glycolysis II (from fructose 6-phosphate)
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glycolysis III (from glucose)
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glycolysis IV (plant cytosol)
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heterolactic fermentation
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Metabolic pathways
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Microbial metabolism in diverse environments
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sucrose biosynthesis I (from photosynthesis)
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superpathway of glucose and xylose degradation
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glycolysis
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SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)
Also acts very slowly on D-glyceraldehyde and some other aldehydes; thiols can replace phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-50-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
strain DSM319
SwissProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
CB1809
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
fragment; strain HM1
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Hippoglossus sp.
halibut
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain ST1
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Manually annotated by BRENDA team
strain ST1
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
MG1363 and relA acid-resistant mutant
-
-
Manually annotated by BRENDA team
commonly known as hed kradang
UniProt
Manually annotated by BRENDA team
commonly known as hed kradang
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain Z2491
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-
Manually annotated by BRENDA team
strain Z2491
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-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
fragment; strain Peru II
UniProt
Manually annotated by BRENDA team
fragment; strain Peru II
UniProt
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
fragment; strain Berok
UniProt
Manually annotated by BRENDA team
fragment; strain Berok
UniProt
Manually annotated by BRENDA team
fragment; strain FVO
UniProt
Manually annotated by BRENDA team
fragment; strain Nilgiri
UniProt
Manually annotated by BRENDA team
fragment; strain Nilgiri
UniProt
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
fragment; strain Uganda I
UniProt
Manually annotated by BRENDA team
fragment; strain Uganda I
UniProt
Manually annotated by BRENDA team
fragment; strain CDC1
UniProt
Manually annotated by BRENDA team
fragment; strain CDC1
UniProt
Manually annotated by BRENDA team
fragment; strain Salvador I
UniProt
Manually annotated by BRENDA team
fragment; strain Salvador I
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain pi9, antarctic psychrophilic bacterium
UniProt
Manually annotated by BRENDA team
strain pi9, antarctic psychrophilic bacterium
UniProt
Manually annotated by BRENDA team
Psithyrus suckleyi
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-
Manually annotated by BRENDA team
European pilchard
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Q97NL1
SwissProt
Manually annotated by BRENDA team
TÜ469, contains pentalenolactone-insensitive glyceraldehyde-3-phosphate dehydrogenase under conditions of production of pentalenolactone. In complex medium no pentalenolactone production is observed and a pentalenolactone-sensitive D-glyceraldehyde 3-phosphate dehydrogenase, rather than the insensitive enzyme can be detected
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Manually annotated by BRENDA team
strain E, ATCC 30005
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Manually annotated by BRENDA team
strain E, ATCC 30005
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Manually annotated by BRENDA team
strain HB 8
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Manually annotated by BRENDA team
under conditions of koningic acid production the koningic-acid-resistant isoenzyme GAPDH I is produced. In peptone-rich medium where non koningic acid is produced the koningic-acid-sensitive isoenzyme GAPDH II is produced in addition to GAPDH II
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Manually annotated by BRENDA team
GAPDH is constitutively expressed
Q4UY27
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-phospho-D-glyceroyl phosphate + NADH
D-glyceraldehyde 3-phosphate + phosphate + NAD+
show the reaction diagram
3-phospho-D-glyceroyl phosphate + NADH + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD+
show the reaction diagram
acetaldehyde + phosphate + NAD+
acetyl phosphate + NADH
show the reaction diagram
acetaldehyde 3-phosphate + NAD+
? + NADH
show the reaction diagram
arsenate + GSH + NAD+ + glyceraldehyde 3-phosphate
arsenite + ?
show the reaction diagram
butyraldehyde + phosphate + NAD+
butyryl phosphate + NADH
show the reaction diagram
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enzyme form E6.6 shows 10% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 15% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 12% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate
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?
D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate
3-phospho-D-glyceroyl phosphate + ?
show the reaction diagram
D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate
3-phospho-D-glyceroyl phosphate + ?
show the reaction diagram
D-glyceraldehyde 3-phosphate + arsenate + NAD+
3-phospho-D-glyceroyl arsenate + NADH
show the reaction diagram
D-glyceraldehyde 3-phosphate + arsenate + NAD+
3-phospho-D-glyceroyl arsenate + NADH + H+
show the reaction diagram
D-glyceraldehyde 3-phosphate + C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+ + phosphate
3-phospho-D-glyceroyl phosphate + C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NADH
show the reaction diagram
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-
-
-
?
D-glyceraldehyde 3-phosphate + N6-(2-carboxyethyl)-NAD+ + phosphate
3-phospho-D-glyceroyl phosphate + N6-(2-carboxyethyl)-NADH
show the reaction diagram
D-glyceraldehyde 3-phosphate + N6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+ + phosphate
3-phospho-D-glyceroyl phosphate + N6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxy))-NADH
show the reaction diagram
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-
-
-
?
D-glyceraldehyde 3-phosphate + NAD+
D-3-phosphoglycerate + NADH
show the reaction diagram
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?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH
show the reaction diagram
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
show the reaction diagram
D-glyceraldehyde 3-phosphate + poly(ethylene glycol)-bound NAD+ + phosphate
3-phospho-D-glyceroyl phosphate + poly(ethylene glycol)-bound NADH
show the reaction diagram
D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate
3-phospho-D-glyceroyl phosphate + thio-NADH
show the reaction diagram
DL-glyceraldehyde + phosphate + NAD+
D-glyceroyl phosphate + NADH
show the reaction diagram
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enzyme form E6.6 shows no activity, enzyme form E6.8 shows 2.5% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 30% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 3.0% of the activity with D-glyceraldehyde 3-phosphate
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?
erythrose 4-phosphate + phosphate + NAD+
? + NADH
show the reaction diagram
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enzyme form E6.6 shows no activity, enzyme form E6.8 shows 1.2% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 25% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 1.5% of the activity with D-glyceraldehyde 3-phosphate
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?
glucose + phosphate + NAD+
? + NADH
show the reaction diagram
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enzyme form E6.6 shows no activity, enzyme form E6.8 shows 0.6% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6.0% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate
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?
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
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?
propionaldehyde + phosphate + NAD+
propionyl phosphate + NADH
show the reaction diagram
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enzyme form E6.6 shows 33% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 12% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 10% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate
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?
valeraldehyde + phosphate + NAD+
pentanoyl phosphate + NADH
show the reaction diagram
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enzyme form E6.6 shows no activity, enzyme form E6.8 shows 19% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 18% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-phospho-D-glyceroyl phosphate + NADH
D-glyceraldehyde 3-phosphate + phosphate + NAD+
show the reaction diagram
-
enzyme plays a central role in glyconeogenesis
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-
?
3-phospho-D-glyceroyl phosphate + NADH + H+
D-glyceraldehyde 3-phosphate + phosphate + NAD+
show the reaction diagram
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH
show the reaction diagram
D-glyceraldehyde 3-phosphate + phosphate + NAD+
3-phospho-D-glyceroyl phosphate + NADH + H+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-acetylpyridine hypoxanthine nucleotide
3-acetylpyridine-NAD+
C6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+
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can replace NAD+ as cofactor
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C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+
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can replace NAD+ as cofactor
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N6-(2-carboxyethyl)-NAD+
NADP+
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wild-type enzyme has no activity with NADP+. The mutant enzyme D32A/L187N shows catalytic efficiency with NADP+ higher than that with NAD+. Activity of mutant enzyme D32A with NAD+ is equal to that of the wild-type enzyme. Activity of mutant L187N with NAD+ is higher than that of the wild-type enzyme. Mutant enzymes D32A and L187N also show activity with NADP+, 3-7% of the activity with NAD+
thio-NAD+
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
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0.045 M, 10fold activation
KCl
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exhibits its highest activity in 2 mol/l KCl at 45°C
Na2SO4
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up to 5fold activation
RbCl
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activity is enhanced by increasing concentrations of the salt. Optimum activity in 3 M RbCl
Zn
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enzyme form E8.5 contains 0.64 gatoms of zinc per mol of enzyme, enzyme form E9.0 contain 2.76 gatom of zinc per mol of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(8E,11E)-C15:2-anacardic acid
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(8E,11E,14E)-C15:3-anacardic acid
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(E)-2-hydroxy-6-(pentadec-8-en-1-yl)benzoic acid
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(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
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treatment with (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide between 0.001 and 1 mM induces the oligomerization of GAPDH, dithiothreitol reduces (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide-induced aggregation in a concentration-dependent manner
1-hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene
2'-deoxy-2'-[(quinolin-7-ylcarbonyl)amino]adenosine
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2,3-diphosphoglycerate
2-(6-amino-2-methyl-9H-purin-9-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
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2-(dodec-1-en-1-yl)-6-hydroxybenzoic acid
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inhibition is not reversed or prevented by addition of Triton X-100. Noncompetitive with respect to both substrate and cofactor
2-(hydroxymethyl)-5-[6-[(2-methylphenyl)amino]-9H-purin-9-yl]tetrahydrofuran-3,4-diol
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2-(hydroxymethyl)-5-[6-[(3-methylbutyl)amino]-9H-purin-9-yl]tetrahydrofuran-3,4-diol
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2-(hydroxymethyl)-5-[6-[(3-methylphenyl)amino]-9H-purin-9-yl]tetrahydrofuran-3,4-diol
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2-methyl-9H-purin-6-amine
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2-pentadecyl-6-hydroxybenzoic acid
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inhibition is not reversed or prevented by addition of Triton X-100. Noncompetitive with respect to both substrate and cofactor
2-[6-amino-8-(pyrimidin-2-ylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
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2-[9-(2-deoxy-2-[[(2,4-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-yl]-2,3-dihydroisoquinoline
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3',4',5',5,7-pentamethoxyflavone
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3',4'-methylenedioxy-5,6,7-trimethoxyflavone
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3',5'-AMP
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3-(1,3-benzodioxol-5-yl)-2-oxo-2H-chromen-6-ylacetate
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3-(1,3-benzodioxol-5-yl)-2-oxo-2H-chromen-7-ylacetate
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3-(1,3-benzodioxol-5-yl)-2-oxo-2H-chromen-8-ylacetate
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3-(1,3-benzodioxol-5-yl)-2H-chromen-2-one
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3-(1,3-benzodioxol-5-yl)-6-hydroxy-2H-chromen-2-one
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3-(1,3-benzodioxol-5-yl)-6-nitro-2H-chromen-2-one
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3-(1,3-benzodioxol-5-yl)-6-[[(1E)-1H-indol-3-ylmethylene]amino]-2H-chromen-2-one
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3-(1,3-benzodioxol-5-yl)-6-[[(1E)-1H-pyrrol-2-ylmethylene]amino]-2H-chromen-2-one
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3-(1,3-benzodioxol-5-yl)-6-[[(1E)-2-furylmethylene]amino]-2H-chromen-2-one
-