Application | Comment | Organism |
---|---|---|
drug development | Gapdh can be an effective target for anti-malarial chemotherapeutics | Plasmodium yoelii |
drug development | Gapdh can be an effective target for anti-malarial chemotherapeutics. Enzyme PfGapdh shows an extra ligand binding capacity in the vicinity of the NAD+ binding region of the active site that may offer an opportunity to design and develop novel antimalarials | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
Gapdh gene is located on chromosome 14, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), addition of 36 amino acid residues (MGS SHHHHHHSSGLVPRGSHMASMTGGNNMGRGSGF-) at the N-terminus of rPfGapdh | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ferriprotoporphyrin IX | strongly inhibits PfGapdh, in contrast to the human GAPDH | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Plasmodium falciparum | |
0.25 | - |
D-glyceraldehyde 3-phosphate | recombinant enzyme, pH and temperature not specified in the publication | Plasmodium falciparum | |
0.28 | - |
NAD+ | recombinant enzyme, pH and temperature not specified in the publication | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | - |
Plasmodium yoelii | - |
- |
cytoskeleton | - |
Plasmodium yoelii | 5856 | - |
cytosol | - |
Plasmodium yoelii | 5829 | - |
additional information | analysis of the subcellular distribution of Gapdh, overview | Plasmodium yoelii | - |
- |
nucleus | - |
Plasmodium yoelii | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
recombinant N-terminally prolongated enzyme, gel filtration | Plasmodium falciparum |
161984 | - |
recombinant N-terminally prolongated enzyme, mass spectrometry | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Plasmodium falciparum | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Plasmodium yoelii | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Plasmodium yoelii 17XL | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
additional information | Plasmodium falciparum | interaction of recombinant PfGapdh with Pfeno, human plasminogen, lysozyme and alpha-tubulin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8T6B1 | - |
- |
Plasmodium yoelii | V7PVE5 | grown in mice | - |
Plasmodium yoelii 17XL | V7PVE5 | grown in mice | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | Plasmodium yoelii, Gapdh undergoes multiple posttranslational modifications | Plasmodium yoelii |
phosphoprotein | multiple phosphorylations changing the pI of the enzyme, overview | Plasmodium yoelii |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme 2.8fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.6 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Plasmodium falciparum | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Plasmodium yoelii | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Plasmodium yoelii 17XL | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
additional information | interaction of recombinant PfGapdh with Pfeno, human plasminogen, lysozyme and alpha-tubulin | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000 + x * 37000 + x * 51000, SDS-PAGE | Plasmodium yoelii |
homotetramer | 4 * 36636, wild-type enzyme, sequence calculation, 4 * 40000, about, recombinant N-terminally prolongated enzyme, SDS-PAGE, 4 * 40355, recombinant N-terminally prolongated enzyme, sequence calculation | Plasmodium falciparum |
More | the 51 kDa enzyme form is present only in the soluble fraction of the cell extract | Plasmodium yoelii |
Synonyms | Comment | Organism |
---|---|---|
glyceraldehyde-3-phosphate dehydrogenase | - |
Plasmodium falciparum |
glyceraldehyde-3-phosphate dehydrogenase | - |
Plasmodium yoelii |
PfGAPDH | - |
Plasmodium falciparum |
PyGapdh | - |
Plasmodium yoelii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Plasmodium yoelii |
9 | - |
recombinant enzyme | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Plasmodium falciparum | |
NAD+ | - |
Plasmodium yoelii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium yoelii | in the cytosolic fraction, variants with mass of about 37 kDa have multiple spots. There are 3-4 spots that have a pI below 8. These spots have a pI of 7.6, 7.2 and 7.0, indicative of multiple phosphorylations, the binding of rPfGapdh with Pfeno, plasminogen, alpha-tubulin and lysozyme is specific | - |
additional information |
Plasmodium falciparum | recombinant wild-type enzyme, sequence calculation and isoelectric focusing | - |
7.77 |
Plasmodium yoelii | sequence calculation | - |
7.95 |
Plasmodium falciparum | recombinant N-terminally prolongated enzyme, sequence calculation and isoelectric focusing | - |
8.33 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme Gapdh is likely to have multiple nonglycolytic functions in the parasite in additon to its function in glycolysis. During intra-erythrocytic phage of its life cycle in humans, the parasite solely relies on glycolysis for its energy needs | Plasmodium falciparum |
physiological function | enzyme Gapdh is likely to have multiple nonglycolytic functions in the parasite in additon to its function in glycolysis. During intra-erythrocytic phage of its life cycle in humans, the parasite solely relies on glycolysis for its energy needs. PfGapdh appearing on the parasite cell surface can bind to extracellular proteins for moonlighting functions due to its specific interactions with with Pfeno, plasminogen, alpha-tubulin and lysozyme | Plasmodium yoelii |