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Literature summary for 1.2.1.12 extracted from
- Multiple enzyme forms of glyceraldehyde-3-phosphate dehydrogenase in Pseudomonas aeruginosa PAO (1987), J. Gen. Microbiol., 133, 3159-3164.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | slight activation | Pseudomonas aeruginosa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-phosphogluconate | - |
Pseudomonas aeruginosa | |
| AMP | - |
Pseudomonas aeruginosa | |
| ATP | - |
Pseudomonas aeruginosa | |
| NADH | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 315000 | - |
non-denaturing PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas aeruginosa | importance of the enzyme in glucose catabolism. Activity is reduced severalfold after growth on gluconeogenic substrates such as citrate compared to growth on glucose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 mM EDTA, 75% glycerol, stable | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pseudomonas aeruginosa | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
| additional information | importance of the enzyme in glucose catabolism. Activity is reduced severalfold after growth on gluconeogenic substrates such as citrate compared to growth on glucose | Pseudomonas aeruginosa | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
5 min, stable | Pseudomonas aeruginosa |
| 55 | - |
5 min, complete loss of activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas aeruginosa | |
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