Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-R3 cells | Homo sapiens |
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystallization of a a highly soluble form of GAPDS truncated at the N-terminus, amino acids 69398. The structure in complex with NAD+ and phosphate maps the two anion-recognition sites within the catalytic pocket that correspond to the conserved Ps site and Pi site identified in other organisms. The structure in complex with NAD+ and glycerol shows serendipitous binding of glycerol at the Ps and Pi sites | Homo sapiens |
in complex with NAD+ and phosphate, hanging drop vapor diffusion method, using 20% (w/v) poly(ethylene glycol) 3350, 0.2 M sodium/potassium phosphate and 10% (v/v) ethylene glycol or in complex with NAD+ and glycerol, hanging drop vapor diffusion method, 20% PEG 3350, 0.2 M Na2SO4, 10% (v/v) ethylene glycol and 0.1 M Bis-Tris propane (pH 6.5) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of a highly soluble form of GAPDS truncated at the N-terminus, amino acids 69398. Mutant displays a 3fold increase in catalytic efficiency and shows homotetrameric structure | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 8.5, 25°C | Homo sapiens | |
0.00046 | - |
D-glyceraldehyde 3-phosphate | N-terminally truncated mutant, pH 8.5, 25°C | Homo sapiens | |
0.035 | - |
NAD+ | N-terminally truncated mutant, pH 8.5, 25°C | Homo sapiens | |
0.1 | - |
NAD+ | wild-type, pH 8.5, 25°C | Homo sapiens | |
0.25 | - |
D-glyceraldehyde 3-phosphate | glyceraldehyde-3-phosphate dehydrogenase, in 10 mM sodium diphosphate, 20 mM sodium phosphate (pH 8.5), 0.003 mM dithiothreitol and 10 mM sodium arsenate, at 25°C | Homo sapiens | |
0.46 | - |
D-glyceraldehyde 3-phosphate | recombinant, highly soluble form of sperm-specific glyceraldehyde-3-phosphate dehydrogenase truncated at the N-terminus, in 10 mM sodium diphosphate, 20 mM sodium phosphate (pH 8.5), 0.003 mM dithiothreitol and 10 mM sodium arsenate, at 25°C | Homo sapiens | |
35 | - |
NAD+ | recombinant, highly soluble form of sperm-specific glyceraldehyde-3-phosphate dehydrogenase truncated at the N-terminus, in 10 mM sodium diphosphate, 20 mM sodium phosphate (pH 8.5), 0.003 mM dithiothreitol and 10 mM sodium arsenate, at 25°C | Homo sapiens | |
100 | - |
NAD+ | glyceraldehyde-3-phosphate dehydrogenase, in 10 mM sodium diphosphate, 20 mM sodium phosphate (pH 8.5), 0.003 mM dithiothreitol and 10 mM sodium arsenate, at 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37672 | - |
4 * 37672, MALDI-TOF mass spectrometry | Homo sapiens |
38000 | - |
4 * 38000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Homo sapiens | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14556 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Superdex S75 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sperm | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 38000, SDS-PAGE | Homo sapiens |
homotetramer | 4 * 37672, MALDI-TOF mass spectrometry | Homo sapiens |
tetramer | N-terminally truncated mutant, crystallization data | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GAPDS | - |
Homo sapiens |
sperm-specific glyceraldehyde-3-phosphate dehydrogenase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
199 | - |
NAD+ | wild-type, pH 8.5, 25°C | Homo sapiens | |
199 | - |
D-glyceraldehyde 3-phosphate | glyceraldehyde-3-phosphate dehydrogenase, in 10 mM sodium diphosphate, 20 mM sodium phosphate (pH 8.5), 0.003 mM dithiothreitol and 10 mM sodium arsenate, at 25°C | Homo sapiens | |
234 | - |
D-glyceraldehyde 3-phosphate | recombinant, highly soluble form of sperm-specific glyceraldehyde-3-phosphate dehydrogenase truncated at the N-terminus, in 10 mM sodium diphosphate, 20 mM sodium phosphate (pH 8.5), 0.003 mM dithiothreitol and 10 mM sodium arsenate, at 25°C | Homo sapiens | |
234 | - |
NAD+ | N-terminally truncated mutant, pH 8.5, 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |