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Literature summary for 1.2.1.12 extracted from
- Heterogeneity of glyceraldehyde-3-phosphate dehydrogenase from human brain (1988), Biochim. Biophys. Acta, 954, 309-324.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E6.8, pH 7 | Homo sapiens |  |
| 0.01 | - |
NAD+ | enzyme form E8.5, pH 9 | Homo sapiens | |
| 0.01 | - |
1,3-diphosphoglyceric acid | enzyme form E6.6, pH 7 | Homo sapiens | |
| 0.012 | - |
1,3-diphosphoglyceric acid | enzyme form E8.5, pH 7 | Homo sapiens | |
| 0.018 | - |
1,3-diphosphoglyceric acid | enzyme form E9.0, pH 7 | Homo sapiens | |
| 0.02 | - |
NAD+ | enzyme form E9.0, pH 9 | Homo sapiens | |
| 0.027 | - |
NAD+ | enzyme form E6.8, pH 9 | Homo sapiens | |
| 0.032 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E9.0, pH 9 | Homo sapiens | |
| 0.035 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E8.5, pH 9 | Homo sapiens | |
| 0.042 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E6.6, pH 9 | Homo sapiens | |
| 0.1 | - |
DL-glyceraldehyde | enzyme form E8.5, pH 7 | Homo sapiens | |
| 0.119 | - |
erythrose 4-phosphate | enzyme form E8.5, pH 7 | Homo sapiens | |
| 0.127 | - |
erythrose 4-phosphate | enzyme form E9.0, pH 7 | Homo sapiens | |
| 0.143 | - |
NAD+ | enzyme form E6.6, pH 9 | Homo sapiens | |
| 0.172 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E6.8, pH 9 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| mitochondrion | enzyme form E6.8, E8.5 and E9.0 | Homo sapiens | 5739 | - |
| additional information | the subcellular localization of enzyme form E6.6 is uncertain | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn | enzyme form E8.5 contains 0.64 gatoms of zinc per mol of enzyme, enzyme form E9.0 contain 2.76 gatom of zinc per mol of enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29500 | - |
4 * 29500, enzyme form E8.5, SDS-PAGE | Homo sapiens |
| 33000 | - |
4 * 33000, enzyme form E9.5, SDS-PAGE | Homo sapiens |
| 36000 | - |
x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE | Homo sapiens |
| 38000 | - |
x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE | Homo sapiens |
| 58000 | - |
1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE | Homo sapiens |
| 61000 | - |
1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE | Homo sapiens |
| 98000 | - |
enzyme form E6.6, gel filtration | Homo sapiens |
| 120000 | - |
enzyme form E8.5, gel filtration | Homo sapiens |
| 133000 | - |
enzyme form E9.0, gel filtration | Homo sapiens |
| 142000 | - |
enzyme form E6.8, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4 enzyme forms: E6.6, E6.8, E8.5 and E9.0 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
enzyme form E6.6 | Homo sapiens |
| 13 | - |
enzyme form E6.8 | Homo sapiens |
| 158 | - |
enzyme form E8.5 | Homo sapiens |
| 620 | - |
enzyme form E9.0 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH | - |
Homo sapiens | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
| acetaldehyde + phosphate + NAD+ | enzyme form E6.6 shows 27% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 9% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.4% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | acetyl phosphate + NADH | - |
? | |
| butyraldehyde + phosphate + NAD+ | enzyme form E6.6 shows 10% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 15% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 12% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | butyryl phosphate + NADH | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Rattus norvegicus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
| DL-glyceraldehyde + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 2.5% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 30% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 3.0% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | D-glyceroyl phosphate + NADH | - |
? | |
| erythrose 4-phosphate + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 1.2% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 25% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 1.5% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | ? + NADH | - |
? | |
| glucose + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 0.6% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6.0% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | ? + NADH | - |
? | |
| propionaldehyde + phosphate + NAD+ | enzyme form E6.6 shows 33% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 12% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 10% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | propionyl phosphate + NADH | - |
? | |
| valeraldehyde + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 19% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 18% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | pentanoyl phosphate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE | Homo sapiens |
| tetramer | 4 * 29500, enzyme form E8.5, SDS-PAGE | Homo sapiens |
| tetramer | x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE | Homo sapiens |
| tetramer | 4 * 33000, enzyme form E9.5, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low | Homo sapiens |
| 8.5 | - |
enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low | Homo sapiens |
| 9.8 | - |
enzyme form E8.5, D-glyceraldehyde 3-phosphate | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Rattus norvegicus | |
| NADH | cofactor | Homo sapiens | |
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