Literature summary for 1.2.1.12 extracted from

Fujita, S.C.; Oshima, T.; Imahori, K.
Purification and properties of D-glyceraldehyde-3-phosphate dehydrogenase from an extreme thermophile, Thermus thermophilus strain HB 8 (1976), Eur. J. Biochem., 64, 57-68.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ethanol	30% v/v, 5fold activation	Thermus thermophilus
propan-1-ol	activates	Thermus thermophilus
propan-2-ol	activates	Thermus thermophilus
tetrabutylammonium chloride	50% activation	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Thermus thermophilus

General Stability

General Stability	Organism
8 M urea, 40% inactivation	Thermus thermophilus
repeated freezing and thawing brings about 10-20% inactivation	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
CdCl2	-	Thermus thermophilus
Cu2+	-	Thermus thermophilus
HgCl2	-	Thermus thermophilus
iodoacetamide	0.5 mM, 9% inhibition	Thermus thermophilus
iodoacetate	0.2 mM, 67% inhibition	Thermus thermophilus
Na2S4O6	0.1 mM, complete inhibition	Thermus thermophilus
NiCl2	-	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	NAD+	-	Thermus thermophilus
0.3	-	D-glyceraldehyde 3-phosphate	-	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
(NH4)2SO4	0.045 M, 10fold activation	Thermus thermophilus
CsCl	activates	Thermus thermophilus
CsCl	maximal activation: 20fold	Thermus thermophilus
K+	maximal activation: 22fold	Thermus thermophilus
LiCl	maximal activation: 10fold	Thermus thermophilus
Na2SO4	up to 5fold activation	Thermus thermophilus
NH4Cl	0.1 M, 25fold activation	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33700	-	4 * 33700, SDS-PAGE	Thermus thermophilus
128000	-	equilibrium sedimentation	Thermus thermophilus
135000	-	gel filtration	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-
Thermus thermophilus HB 8	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.4	-	-	Thermus thermophilus

Storage Stability

Storage Stability	Organism
-20°C, stable for at least 2 years	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Thermus thermophilus	3-phospho-D-glyceroyl phosphate + NADH	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Thermus thermophilus HB 8	3-phospho-D-glyceroyl phosphate + NADH	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 33700, SDS-PAGE	Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
85	-	pH 8.5, protein concentration 2.1 mg/ml, 10 min, stable	Thermus thermophilus
90	-	pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 20% loss of acticity	Thermus thermophilus
95	-	pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 85% loss of acticity	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	-	Thermus thermophilus

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	8.8	pH 7.0: about 55% of maximal activity, pH 8.8: about 95% of maximal activity	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermus thermophilus

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Release 2023.1 (January 2023)