Any feedback?
Literature summary for 1.2.1.12 extracted from
- Glyceraldehyde phosphate dehydrogenase of Escherichia coli. Structural and catalytic properties (1971), J. Biol. Chem., 246, 4326-4333.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| after addition of NAD+ to the apoprotein | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli |  |
| AMP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
| ATP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
| iodoacetate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Escherichia coli | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Escherichia coli | p-nitrophenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
50 mM Tris-chloride buffer and triethanolamine-chloride buffer | Escherichia coli |
| 8.8 | - |
50 mM sodium diphosphate buffer | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | about 85% of maximal activity at pH 7.5 and pH 8.5, 50 mM Tris-chloride buffer. About 75% of maximal activity at pH 7.5 and pH 8.5, 50 mM triethanolamine-chloride buffer | Escherichia coli |
| 8.3 | 9.3 | 85% of maximal activity at pH 8.3 and pH 9.3, 50 mM sodium diphosphate buffer | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | contains less than 0.3 mol of NAD+ per mol of native protein | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
