Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21DE | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C153S | mutant, having only one cysteine in the catalytic site, is oxidized and glutathionylated similarly to the wild-type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 1 mM | Arabidopsis thaliana | |
Diamide | 1 mM | Arabidopsis thaliana | |
glutathione | 5 mM oxidized glutathione, GSSG, formation of mixed disulfide between glutathione and A4-GAPDH results in the inhibition of enzyme activity | Arabidopsis thaliana | |
glutathione | formation of mixed disulfide between glutathione and GAPDH results in the inhibition of enzyme activity | Spinacia oleracea | |
H2O2 | 1 mM | Arabidopsis thaliana | |
H2O2 | - |
Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36140 | - |
theoretical mass of the A subunit | Spinacia oleracea |
39360 | - |
theoretical mass of the B subunit | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH + H+ | Spinacia oleracea | - |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
3-phospho-D-glyceroyl phosphate + NADH + H+ | Arabidopsis thaliana | - |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glutathionylation | - |
Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
native GAPDH is purified from spinach chloroplasts | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Spinacia oleracea | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Arabidopsis thaliana | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | A2B2-GAPDH, light conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation | Spinacia oleracea |
homotetramer | A4-GAPDH | Arabidopsis thaliana |
multimer | A8B8-GAPDH, dark conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
A4-GAPDH | - |
Arabidopsis thaliana |
A4-glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
AnBn-GAPDH | - |
Spinacia oleracea |
GAPDH | - |
Spinacia oleracea |
GAPDH | - |
Arabidopsis thaliana |
glyceraldehyde-3-phosphate dehydrogenase | - |
Spinacia oleracea |
glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
activity assay | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Spinacia oleracea | |
NADH | - |
Arabidopsis thaliana | |
NADPH | - |
Spinacia oleracea |