Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-blue cells transformed with pNeis2 | Neisseria meningitidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | strong inhibition at 10 mM | Neisseria meningitidis | |
dithiothreitol | strong inhibition at 2 mM | Neisseria meningitidis | |
additional information | NADH, glucose-1-phosphate, AMP, ADP, ATP, and fructose-6-phosphate do not affect kinetic properties of GAPN and no change in cofactor preference from NAD+ to NADP+ in the presence of these metabolic intermediates is detected | Neisseria meningitidis | |
NADP+ | 75% inhibition in the presence of 2 mM NADP+ | Neisseria meningitidis | |
NADPH | - |
Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
NAD+ | recombinant enzyme, in the presence of D-glyceraldehyde 3-phosphate, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis | |
0.088 | - |
NAD+ | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), in the presence of glyceraldehyde, at 25°C | Neisseria meningitidis | |
0.109 | - |
NAD+ | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), in the presence of acetaldehyde, at 25°C | Neisseria meningitidis | |
0.2 | - |
glyceraldehyde | recombinant enzyme, at 25°C | Neisseria meningitidis | |
0.31 | - |
acetaldehyde | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis | |
1.45 | - |
D-glyceraldehyde 3-phosphate | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Neisseria meningitidis |
51000 | - |
4 * 51000, native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS | Neisseria meningitidis |
200000 | - |
SDS-PAGE | Neisseria meningitidis |
208000 | - |
native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS | Neisseria meningitidis |
209200 | - |
calculated from amino acid sequence | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Neisseria meningitidis Z2491 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose DE-52 column chromatography, and Cibacron Blue Sepharose column chromatography | Neisseria meningitidis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.52 | - |
recombinant enzyme from crude extract, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis |
2.16 | - |
crude extract, at 25°C | Neisseria meningitidis |
25.1 | - |
recombinant enzyme after 48.3fold purification, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + phosphate + NAD+ | - |
Neisseria meningitidis | acetyl phosphate + NADH | - |
? | |
acetaldehyde + phosphate + NAD+ | - |
Neisseria meningitidis Z2491 | acetyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + NAD+ | - |
Neisseria meningitidis | D-3-phosphoglycerate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Neisseria meningitidis | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Neisseria meningitidis Z2491 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
glyceraldehyde + NAD+ + H2O | - |
Neisseria meningitidis | glycerate + NADH + H+ | - |
? | |
glyceraldehyde + NAD+ + H2O | - |
Neisseria meningitidis Z2491 | glycerate + NADH + H+ | - |
? | |
glyceraldehyde 3-phosphate + NAD+ | - |
Neisseria meningitidis | ? + NADH | - |
? | |
glyceraldehyde 3-phosphate + NAD+ | - |
Neisseria meningitidis Z2491 | ? + NADH | - |
? | |
additional information | no activity with NADP+ | Neisseria meningitidis | ? | - |
? | |
additional information | no activity with NADP+ | Neisseria meningitidis Z2491 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Neisseria meningitidis |
homotetramer | 4 * 51000, native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Neisseria meningitidis |
GAPN | - |
Neisseria meningitidis |
NAD-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase | - |
Neisseria meningitidis |
NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
64 | - |
- |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.03 | - |
acetaldehyde | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis | |
4.35 | - |
glyceraldehyde | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis | |
24.51 | - |
D-glyceraldehyde 3-phosphate | recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25°C | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Neisseria meningitidis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | undetectable activity with NADP+ | Neisseria meningitidis | |
NAD+ | - |
Neisseria meningitidis | |
NAD+ | absolute specificity for NAD+ | Neisseria meningitidis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Neisseria meningitidis | isoelectric focusing | - |
6.3 |