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Literature summary for 1.2.1.12 extracted from
- Cloning, expression and characterization of NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase of adult Haemonchus contortus (2011), J. Helminthol., 85, 421-429.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Haemonchus contortus |
| expressed in Escherichia coli BL21(DE3) cells | Haemonchus contortus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36446 | - |
x * 36446, calculated from amino acid sequence | Haemonchus contortus |
| 36446 | - |
x * 36446, calculated, x * 38000, SDS-PAGE | Haemonchus contortus |
| 38000 | - |
x * 38000, SDS-PAGE | Haemonchus contortus |
| 38000 | - |
x * 36446, calculated, x * 38000, SDS-PAGE | Haemonchus contortus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Haemonchus contortus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemonchus contortus | - |
- |
- |
| Haemonchus contortus | D9IL10 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no signal peptide is found in the deduced protein | Haemonchus contortus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Haemonchus contortus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adult | - |
Haemonchus contortus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 46 | - |
pH 8.0, 40°C | Haemonchus contortus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Haemonchus contortus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Haemonchus contortus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Haemonchus contortus |
| ? | x * 36446, calculated from amino acid sequence | Haemonchus contortus |
| ? | x * 36446, calculated, x * 38000, SDS-PAGE | Haemonchus contortus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAPDH | - |
Haemonchus contortus |
| NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Haemonchus contortus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Haemonchus contortus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | - |
Haemonchus contortus |
| 20 | 45 | temperatures varying between 20 and 45°C do not greatly affect enzymatic activity. However, thermal inactivation occurs above 45°C and results in a total loss of activity at 75°C | Haemonchus contortus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
the enzyme maintains activity at high temperatures of up to 75°C | Haemonchus contortus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Haemonchus contortus |
| 8 | - |
when the pH is above 8.0, the enzyme displays a sharp decrease in catalytic activity. However, when the pH is below 8.0, the enzymatic activity does not decrease so sharply | Haemonchus contortus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
sharp decrease in activity above | Haemonchus contortus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Haemonchus contortus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Haemonchus contortus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Haemonchus contortus | calculated | - |
8.2 |
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