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EC 1.14.13.162 Details
show Brenda entryEC number
1.14.13.162
Accepted name
2,5-diketocamphane 1,2-monooxygenase
Reaction
(+)-bornane-2,5-dione + O2 + NADH + H+ = (+)-5-oxo-1,2-campholide + NAD+ + H2O
Other name(s)
2,5-diketocamphane lactonizing enzyme,
ketolactonase I (ambiguous),
2,5-diketocamphane 1,2-monooxygenase oxygenating component,
2,5-DKCMO,
camP (gene name),
camphor 1,2-monooxygenase,
camphor ketolactonase I
Systematic name
(+)-bornane-2,5-dione,NADH:oxygen oxidoreductase (1,2-lactonizing)
Comment
A flavoprotein (FMN) which requires Fe2+. A Baeyer-Villiger monooxygenase isolated from camphor-grown strains of Pseudomonas putida and encoded on the cam plasmid. Involved in the degradation of (+)-camphor. Requires a dedicated NADH-FMN reductase [cf. EC 1.5.1.42, FMN reductase (NADH)] [1-3]. Can accept several bicyclic ketones including (+)- and (–)-camphor [6] and adamantanone [4]. The product spontaneously converts to [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate.
History
created 1972 as EC 1.14.15.2, transferred 2012 to EC 1.14.13.162, deleted 2018
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.6.4.1 created 1961, deleted 2002 1.6.4.2 created 1961, modified 1989, deleted 2002 1.6.4.3 created 1961, modified 1976, deleted 1983 1.6.4.4 created 1965, deleted 2002 1.6.4.5 created 1972, deleted 2002 1.6.4.6 created 1972, deleted 2002 1.6.4.7 created 1978, deleted 2002 1.6.4.8 created 1989, deleted 2002 1.6.4.9 created 1992, deleted 2002 1.6.4.10 created 1992, deleted 2002 1.14.13.45 created 1992, deleted 2003
